Antibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Other assay [2]
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Validation data
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- Product number
- 34-5400 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Claudin 16 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.25 mg/mL
- Storage
- -20°C
Submitted references Claudin-7, -16, and -19 during mouse kidney development.
Tight junctional localization of claudin-16 is regulated by syntaxin 8 in renal tubular epithelial cells.
Enhanced immunohistochemical resolution of claudin proteins in glycolmethacrylate-embedded tissue biopsies.
Differential expression of claudin tight junction proteins in the human cortical nephron.
Claudin-1 has tumor suppressive activity and is a direct target of RUNX3 in gastric epithelial cells.
Endothelia of term human placentae display diminished expression of tight junction proteins during preeclampsia.
Role of claudin interactions in airway tight junctional permeability.
Khairallah H, El Andalousi J, Simard A, Haddad N, Chen YH, Hou J, Ryan AK, Gupta IR
Tissue barriers 2014;2(4):e964547
Tissue barriers 2014;2(4):e964547
Tight junctional localization of claudin-16 is regulated by syntaxin 8 in renal tubular epithelial cells.
Ikari A, Tonegawa C, Sanada A, Kimura T, Sakai H, Hayashi H, Hasegawa H, Yamaguchi M, Yamazaki Y, Endo S, Matsunaga T, Sugatani J
The Journal of biological chemistry 2014 May 9;289(19):13112-23
The Journal of biological chemistry 2014 May 9;289(19):13112-23
Enhanced immunohistochemical resolution of claudin proteins in glycolmethacrylate-embedded tissue biopsies.
Collins JE, Kirk A, Campbell SK, Mason J, Wilson SJ
Methods in molecular biology (Clifton, N.J.) 2011;762:371-82
Methods in molecular biology (Clifton, N.J.) 2011;762:371-82
Differential expression of claudin tight junction proteins in the human cortical nephron.
Kirk A, Campbell S, Bass P, Mason J, Collins J
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 2010 Jul;25(7):2107-19
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 2010 Jul;25(7):2107-19
Claudin-1 has tumor suppressive activity and is a direct target of RUNX3 in gastric epithelial cells.
Chang TL, Ito K, Ko TK, Liu Q, Salto-Tellez M, Yeoh KG, Fukamachi H, Ito Y
Gastroenterology 2010 Jan;138(1):255-65.e1-3
Gastroenterology 2010 Jan;138(1):255-65.e1-3
Endothelia of term human placentae display diminished expression of tight junction proteins during preeclampsia.
Liévano S, Alarcón L, Chávez-Munguía B, González-Mariscal L
Cell and tissue research 2006 Jun;324(3):433-48
Cell and tissue research 2006 Jun;324(3):433-48
Role of claudin interactions in airway tight junctional permeability.
Coyne CB, Gambling TM, Boucher RC, Carson JL, Johnson LG
American journal of physiology. Lung cellular and molecular physiology 2003 Nov;285(5):L1166-78
American journal of physiology. Lung cellular and molecular physiology 2003 Nov;285(5):L1166-78
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of Hep G2 (Lane 1) and A549 (Lane 2). The blot was probed with Anti-Claudin-16 Rabbit Polyclonal Antibody (Product # 34-5400, 2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 34 kDa band corresponding to Claudin 16 was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Claudin 16 was performed using 90% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Claudin 16 Rabbit Polyclonal Antibody (Product # 34-5400) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing junctional localization. Panel e shows the control without primary antibody. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL