34-5400

antibody from Invitrogen Antibodies

Targeting: CLDN16 HOMG3, PCLN1

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

34-5400

Provider

Invitrogen Antibodies

Product name

Claudin 16 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

0.25 mg/mL

Storage

-20°C

References

Submitted references

Claudin-7, -16, and -19 during mouse kidney development.

Khairallah H, El Andalousi J, Simard A, Haddad N, Chen YH, Hou J, Ryan AK, Gupta IR
Tissue barriers 2014;2(4):e964547

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Tight junctional localization of claudin-16 is regulated by syntaxin 8 in renal tubular epithelial cells.

Ikari A, Tonegawa C, Sanada A, Kimura T, Sakai H, Hayashi H, Hasegawa H, Yamaguchi M, Yamazaki Y, Endo S, Matsunaga T, Sugatani J
The Journal of biological chemistry 2014 May 9;289(19):13112-23

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Enhanced immunohistochemical resolution of claudin proteins in glycolmethacrylate-embedded tissue biopsies.

Collins JE, Kirk A, Campbell SK, Mason J, Wilson SJ
Methods in molecular biology (Clifton, N.J.) 2011;762:371-82

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Differential expression of claudin tight junction proteins in the human cortical nephron.

Kirk A, Campbell S, Bass P, Mason J, Collins J
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 2010 Jul;25(7):2107-19

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Claudin-1 has tumor suppressive activity and is a direct target of RUNX3 in gastric epithelial cells.

Chang TL, Ito K, Ko TK, Liu Q, Salto-Tellez M, Yeoh KG, Fukamachi H, Ito Y
Gastroenterology 2010 Jan;138(1):255-65.e1-3

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Endothelia of term human placentae display diminished expression of tight junction proteins during preeclampsia.

Liévano S, Alarcón L, Chávez-Munguía B, González-Mariscal L
Cell and tissue research 2006 Jun;324(3):433-48

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Role of claudin interactions in airway tight junctional permeability.

Coyne CB, Gambling TM, Boucher RC, Carson JL, Johnson LG
American journal of physiology. Lung cellular and molecular physiology 2003 Nov;285(5):L1166-78

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of Claudin 16 was performed using 90% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Claudin 16 Rabbit Polyclonal Antibody (Product # 34-5400) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing junctional localization. Panel e shows the control without primary antibody. The images were captured at 60X magnification.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL