Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- PA5-57817 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HAT1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: KFKINKQHAR RVYEILRLLV TDMSDAEQYR SYRLDIKRRL ISPYKKKQRD LAKMRKCLRP EELTNQMNQI EISMQHEQLE ESFQELVEDY RRVIERLA Highest antigen sequence identity to the following orthologs: Mouse - 96%, Rat - 96%.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Cell cycle-resolved chromatin proteomics reveals the extent of mitotic preservation of the genomic regulatory landscape.
Ginno PA, Burger L, Seebacher J, Iesmantavicius V, Schübeler D
Nature communications 2018 Oct 2;9(1):4048
Nature communications 2018 Oct 2;9(1):4048
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HAT1 in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using a HAT1 Polyclonal Antibody (Product # PA5-57817). Remaining relative intensity is presented. Loading control: Anti-PPIB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HAT1 in mouse cell line NIH-3T3 and rat cell line NBT-II using a HAT1 Polyclonal Antibody (Product # PA5-57817).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HAT1 in human cell line U-2 OS shows positivity in nucleus but excluded from the nucleoli. Samples were probed using a HAT1 Polyclonal Antibody (Product # PA5-57817).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of HAT1 in human lymph node shows strong nuclear positivity in germinal center cells. Samples were probed using a HAT1 Polyclonal Antibody (Product # PA5-57817).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of HAT1 in human lymph node and pancreas tissues using HAT1 Polyclonal Antibody (Product # PA5-57817). Corresponding HAT1 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of HAT1 in human pancreas using HAT1 Polyclonal Antibody (Product # PA5-57817) shows low expression as expected.