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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-29174 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HAT1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Recommended positive controls: U87-MG.
- Concentration
- 0.98 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HAT1 using 30 µg of U87-MG lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a HAT1 polyclonal antibody (Product # PA5-29174) at a dilution of 1:10,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HAT1 Polyclonal Antibody detects HAT1 protein by Western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with HAT1 Polyclonal Antibody (Product # PA5-29174) diluted by 1:10,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HAT1 Polyclonal Antibody detects HAT1 protein by Western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with HAT1 Polyclonal Antibody (Product # PA5-29174) diluted by 1:10,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HAT1 showing staining in the cytoplasm and nucleus of HeLa cells. HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a HAT1 polyclonal antibody (Product # PA5-29174) diluted at 1:500. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HAT1 Polyclonal Antibody [N1C3-2] detects HAT1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HAT1 protein stained by HAT1 Polyclonal Antibody [N1C3-2] (Product # PA5-29174) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon, using HAT1 (Product # PA5-29174) antibody at 1:250 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HAT1 Polyclonal Antibody immunoprecipitates HAT1 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 µg HeLa whole cell lysate/extract B. Control with 2 µg of preimmune rabbit IgG C. Immunoprecipitation of HAT1 protein by 2 µg of HAT1 antibody (Product # PA5-29174) 12% SDS-PAGE The immunoprecipitated HAT1 protein was detected by HAT1 Polyclonal Antibody (Product # PA5-29174) diluted at 1:1,000.
