Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
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Validation data
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- Product number
- PA5-120632 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GPR22 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Positive test controls include: U-87MG, U-251MG, SH-SY5Y, HepG2, Mouse heart, Mouse brain, Rat heart, Rat brain. Immunogen sequence: MCFSPILEIN MQSESNITVR DDIDDINTNM YQPLSYPLSF QVSLTGFLML EIVLGLGSNL TVLVLYCMKS NLINSVSNII TMNLHVLDVI ICVGCIPLTI
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 6.34 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of GPR22 in various cell lines using a GPR22 Polyclonal antibody (Product # PA5-120632) at a dilution of 1:1,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performed for 90s.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of GPR22 in L929 cells using a GPR22 Polyclonal antibody (Product # PA5-120632). Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of GPR22 in L929 cells using a GPR22 Polyclonal antibody (Product # PA5-120632). Blue: DAPI for nuclear staining.