Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- MA5-23870 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ADAM8 Monoclonal Antibody (143303)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- In Western blots, less than 5% cross-reactivity with the ectodomains of recombinant human ADAM9, 10, 15, 17, and 19 is observed. This antibody detects an epitope within aa 201-497 of recombinant human ADAM8 which corresponds to the metalloprotease and disintegrin-like domains. Reconstitute at 0.5 mg/mL in sterile PBS. Endoxin level is
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 143303
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of ADAM8 in recombinant human ADAM8 aa 158-497 . Samples were incubated in ADAM8 monoclonal antibody (Product # MA5-23870) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Mouse IgG secondary antibody. A specific band was detected for ADAM8 aa158-497 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions.