10469-1-AP

antibody from Proteintech Group

Targeting: RAN ARA24, Gsp1, TC4

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

10469-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#10469-1-AP, RRID:AB_2176484

Product name

RAN antibody

Antibody type

Polyclonal

Description

RAN antibody (Cat. #10469-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

GEF-H1 upon microtubule destabilization drives ZBP1-dependent necroptosis.

Zhang X, Li X, Yang Y, Wu J, Liu Q, Deng L, Huang J, Zhao Y, Ren J, Wu X
Cell death and differentiation 2026 Mar 17;

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Lactylation-driven molecular taxonomy of melanoma: linking epigenetic modifications to immune evasion and clinical outcomes.

Wang L, Jin XY, Zhu LY, Wu YC, Qiu RN, Feng LF, Jin HC, Wang JF
American journal of cancer research 2025;15(7):2911-2931

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H3K36me3-Guided m(6)A Modification of Oncogenic L1CAM-AS1 Drives Macrophage Polarization and Immunotherapy Resistance in Hepatocellular Carcinoma.

Wang T, Han L, Huo Y, Zhang L, Huang Y, Zhang N, Yang M
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 Sep;12(33):e14909

---

FSH-Induced Nuclear Exclusion of FOXO1 Mediated by PI3K/Akt Signaling Pathway in Granulosa Cells Is Associated with Follicle Selection and Growth of the Hen Ovary.

Yan C, Ou Y, Sun X, Sun Y, Zhao J, Qin N, Xu R
Cells 2025 Nov 26;14(23)

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A SUMO-interacting motif in the guanine nucleotide exchange factor EPAC1 is required for subcellular targeting and function.

Yang W, Mei F, Lin W, Lee JE, Nie S, Bley CJ, Hoelz A, Cheng X
bioRxiv : the preprint server for biology 2025 May 3;

---

A SUMO-interacting motif in the guanine nucleotide exchange factor EPAC1 is required for subcellular targeting and function.

Yang W, Mei F, Lin W, Lee JE, Nie S, Bley CJ, Hoelz A, Cheng X
The Journal of biological chemistry 2025 Jun;301(6):110279

---

Neonatal diabetes-associated missense PDX1 variant disrupts chromatin association and protein-protein interaction.

Yang X, Zanfardino A, Schiaffini R, Ishibashi J, Daniel B, Haemmerle MW, Rapini N, Piscopo A, Miraglia Del Giudice E, Digilio MC, Iorio R, Mucciolo M, Cianfarani S, Iafusco D, Barbetti F, Stoffers DA
JCI insight 2025 Jun 9;10(11)

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Kinesin KIF16B Participates in G2/M Transition and Microtubule Dynamics via Aurora A-PLK1 in Oocyte Meiosis.

Li MX, Zhang KH, Zou YJ, Lu PS, Sun SC, Wang Y
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2025 Jul 31;39(14):e70854

---

Proteome of oocyte spindle identifies Ccdc69 regulates spindle assembly like "band-tightening spell".

Guo JN, Zhu L, Meng TG, Zhang YN, Sun SM, Yang XM, Zhao BW, Lu YK, Xu YH, Yue W, Han Z, Wong CCL, Wang ZB
Cellular and molecular life sciences : CMLS 2025 Jul 30;82(1):292

---

Pathogenic variants of TUBB8 cause oocyte spindle defects by disrupting with EB1/CAKP5 interactions and potential treatment targeting microtubule acetylation through HDAC6 inhibition.

Luo H, Chen J, Li C, Wu T, Yin S, Yang G, Wang Y, Guo Z, Hu S, He Y, Wang Y, Chen Y, Su Y, Miao C, Qian Y, Feng R
Clinical and translational medicine 2025 Jan;15(1):e70193

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E3 ligase substrate adaptor SPOP fine-tunes the UPR of pancreatic β cells.

Oguh AU, Haemmerle MW, Sen S, Rozo AV, Shrestha S, Cartailler JP, Fazelinia H, Ding H, Preza S, Yang J, Yang X, Sussel L, Alvarez-Dominguez JR, Doliba N, Spruce LA, Arrojo E Drigo R, Stoffers DA
Genes & development 2025 Feb 3;39(3-4):261-279

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RNA binding proteins PCBP1 and PCBP2 regulate pancreatic β cell translation.

Haemmerle MW, Batmanov K, Sen S, Varney MJ, Utecht AT, Good AL, Scota AV, Tersey SA, Ghanem LR, Philpott CC, Stoffers DA
Molecular metabolism 2025 Aug;98:102175

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Nuclear proteasomes buffer cytoplasmic proteins during autophagy compromise.

Park SJ, Son SM, Barbosa AD, Wrobel L, Stamatakou E, Squitieri F, Balmus G, Rubinsztein DC
Nature cell biology 2024 Oct;26(10):1691-1699

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Thermal proteome profiling reveals fructose-1,6-bisphosphate as a phosphate donor to activate phosphoglycerate mutase 1.

Zhang Y, Cao Y, Wu X, Chen Z, Chen B, Wang A, Guo Y, Chen W, Xue R, Liu Z, Li Y, Li T, Cheng R, Zhou N, Li J, Liu Y, Zhao X, Luo H, Xu M, Li H, Geng Y
Nature communications 2024 Oct 16;15(1):8936

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Arf1 GTPase Regulates Golgi-Dependent G2/M Transition and Spindle Organization in Oocyte Meiosis.

Zhang KH, Zou YJ, Shan MM, Pan ZN, Ju JQ, Liu JC, Ji YM, Sun SC
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2024 Jan;11(4):e2303009

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The noncanonical RNA-binding protein RAN stabilizes the mRNA of intranuclear stress granule assembly factor G3BP1 in nasopharyngeal carcinoma.

Yang PY, Yang Z, Lv J, Jiang PY, Quan TQ, Huang ZH, Xu XD, Guo R, Wei D, Sun Y
The Journal of biological chemistry 2024 Dec;300(12):107964

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Inhibition of RAN attenuates influenza a virus replication and nucleoprotein nuclear export.

Cao L, She Z, Zhao Y, Cheng C, Li Y, Xu T, Mao H, Zhang Y, Hui X, Lin X, Wang T, Sun X, Huang K, Zhao L, Jin M
Emerging microbes & infections 2024 Dec;13(1):2387910

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Structural basis for a nucleoporin exportin complex between RanBP2, SUMO1-RanGAP1, the E2 Ubc9, Crm1 and the Ran GTPase.

Baytshtok V, DiMattia MA, Lima CD
bioRxiv : the preprint server for biology 2024 Dec 26;

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The survivin-ran inhibitor LLP-3 decreases oxidative phosphorylation, glycolysis and growth of neuroblastoma cells.

Galiger C, Zohora FT, Dorneburg C, Tews D, Debatin KM, Beltinger C
BMC cancer 2023 Nov 25;23(1):1148

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A novel T-cell proliferation-associated gene predicts prognosis and reveals immune infiltration in patients with oral squamous cell carcinoma.

Cui Y, Cheng Y, Huang W, Liu J, Zhang X, Bu M, Li X
Archives of oral biology 2023 Aug;152:105719

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The mechanism of acentrosomal spindle assembly in human oocytes.

Wu T, Dong J, Fu J, Kuang Y, Chen B, Gu H, Luo Y, Gu R, Zhang M, Li W, Dong X, Sun X, Sang Q, Wang L
Science (New York, N.Y.) 2022 Nov 18;378(6621):eabq7361

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RSL1D1 promotes the progression of colorectal cancer through RAN-mediated autophagy suppression.

Liu X, Chen J, Long X, Lan J, Liu X, Zhou M, Zhang S, Zhou J
Cell death & disease 2022 Jan 10;13(1):43

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A new candidate oncogenic lncRNA derived from pseudogene WFDC21P promotes tumor progression in gastric cancer.

Cui H, Jiang Z, Zeng S, Wu H, Zhang Z, Guo X, Dong K, Wang J, Shang L, Li L
Cell death & disease 2021 Oct 2;12(10):903

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Saturated fatty acids entrap PDX1 in stress granules and impede islet beta cell function.

Zhang M, Yang C, Zhu M, Qian L, Luo Y, Cheng H, Geng R, Xu X, Qian C, Liu Y
Diabetologia 2021 May;64(5):1144-1157

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A nuclear export sequence promotes CRM1-dependent targeting of the nucleoporin Nup214 to the nuclear pore complex.

Hamed M, Caspar B, Port SA, Kehlenbach RH
Journal of cell science 2021 Mar 26;134(6)

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Lin28A Regulates Stem-like Properties of Ovarian Cancer Cells by Enriching RAN and HSBP1 mRNA and Up-regulating its Protein Expression.

Zhong Y, Cao L, Ma H, Wang Q, Wei P, Yang J, Mo Y, Cao L, Shuai C, Peng S
International journal of biological sciences 2020;16(11):1941-1953

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Doublecortin undergo nucleocytoplasmic transport via the RanGTPase signaling to promote glioma progression.

Ayanlaja AA, Ji G, Wang J, Gao Y, Cheng B, Kanwore K, Zhang L, Xiong Y, Kambey PA, Gao D
Cell communication and signaling : CCS 2020 Feb 12;18(1):24

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SIRT7 regulates the nuclear export of NF-κB p65 by deacetylating Ran.

Sobuz SU, Sato Y, Yoshizawa T, Karim F, Ono K, Sawa T, Miyamoto Y, Oka M, Yamagata K
Biochimica et biophysica acta. Molecular cell research 2019 Sep;1866(9):1355-1367

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Identification and function analysis of ras-related nuclear protein from Macrobrachium rosenbergii involved in Spiroplasma eriocheiris infection.

Ning M, Xiu Y, Yuan M, Bi J, Liu M, Wei P, Yan Y, Gu W, Wang W, Meng Q
Fish & shellfish immunology 2017 Nov;70:583-592

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Identification of nasopharyngeal carcinoma metastasis-related biomarkers by iTRAQ combined with 2D-LC-MS/MS.

Chen Z, Long L, Wang K, Cui F, Zhu L, Tao Y, Wu Q, Xiang M, Liang Y, Qiu S, Xiao Z, Yi B
Oncotarget 2016 Jun 7;7(23):34022-37

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 10469-1-AP(RAN antibody) at dilution of 1:1000

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human testis using 10469-1-AP(RAN antibody) at dilution of 1:100 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The RAN antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human RAN. This antibody recognizes human, mouse, rat antigen. The RAN antibody has been validated for the following applications: ELISA, WB, IHC analysis.

Appendix

10469-1-AP.pdf

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