Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- BS1308 - Provider product page
- Provider
- Bioworld Technology, Inc
- Proper citation
- Bioworld Technology Cat#BS1308, RRID:AB_1663456
- Product name
- RAN (E202) polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide, corresponding to amino acids 170-200 of Human RAN.
- Description
- The small Ras-related protein Ran, also called TC4, is a 24 kDa, nuclear localized GTPase implicated in a diverse array of cellular processes including DNA replication, entry into and exit from mitosis and the transport of RNA and proteins through the nuclear pore complex. Like Ras, active Ran GTP and inactive Ran GDP levels are tightly regulated by guanine nucleotide exchange factors (GEFs) and GTPase activating proteins (GAPs). The abundant GEF, RCC1 (regulator of chromosome condensation 1), increases the rate at which Ran exchanges GDP for GTP. Ran GAP1 opposes the effects of RCC1 by increasing the rate at which Ran hydrolyzes GTP to GDP. A 23 kDa protein designated Ran BP1 has no intrinsic GAP activity, and functions as a GEF inhibitor deactivating RCC1 and thereby indirectly increasing the ratio of Ran GDP to Ran GTP. The 358 kDa protein Ran BP2 has been proposed as the Ran GTP docking site at the periphery of the nuclear pore complex.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Bioworld Technology, Inc (provider)
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- Experimental details
- Western blot (WB) analysis of RAN (E202) pAb at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:sp2/0 whole cell lysateLane3:H9C2 whole cell lysate