PA5-29926

antibody from Invitrogen Antibodies

Targeting: RAN ARA24, Gsp1, TC4

Antibody data

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Validation data

RAN protein structure - PA5-29926 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of RAN in Mouse tissue extracts (50 µg). Samples was separated by 12% SDS-PAGE and the membrane was probed with RAN Polyclonal antibody (Product # PA5-29926) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on whole cell extracts (30 µg lysate) of NIH/3T3 (Lane 1), Jurkat (Lane 2), U-87 MG (Lane 3) and Hep G2 (Lane 4). The blot was probed with Anti-RAN Polyclonal Antibody (Product # PA5-29926, 1:500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 24 kDa band corresponding to RAN was observed across the cell lines tested.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of RAN was achieved by transfecting HeLa with RAN specific siRNAs (Silencer® select Product # s11769 ). Western blot analysis (Fig. a) was performed using whole cell extracts from the RAN knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with RAN Polyclonal Antibody (Product # PA5-29926, 1:500 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to RAN.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using RAN Polyclonal Antibody (Product # PA5-29926). Sample (30 µg of whole cell lysate). Lane A: HeLa. 12% SDS PAGE. RAN Polyclonal Antibody (Product # PA5-29926) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: RAN Polyclonal Antibody detects RAN protein by western blot analysis. Mouse tissue extracts (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with RAN Polyclonal Antibody (Product # PA5-29926) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.