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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [7]
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- Product number
- PA5-53836 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HOOK1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: KRALEELQEA LAEKEELRQR CEELDMQVTT LQDEKNSLVS ENEMMNEKLD QLDGSFDDPN TVVAKKYFHA QLQLEQLQEE NFRLEAAKDD YRVHC
- Concentration
- 0.3 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HOOK1 in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells). Samples were probed using a HOOK1 Polyclonal Antibody (Product # PA5-53836).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HOOK1 in Lane 1: Marker (kDa) 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp; Lane 4: Human plasma (IgG/HSA depleted); Lane 5: Human liver tissue.; Lane 6: Human tonsil tissue. tissue. Samples were probed using a HOOK1 Polyclonal Antibody (Product # PA5-53836).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of HOOK1 in human colon using HOOK1 Polyclonal Antibody (Product # PA5-53836).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of HOOK1 in human liver using HOOK1 Polyclonal Antibody (Product # PA5-53836).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of HOOK1 in human lymph node using HOOK1 Polyclonal Antibody (Product # PA5-53836).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of HOOK1 in human testis and endometrium tissues using HOOK1 Polyclonal Antibody (Product # PA5-53836). Corresponding HOOK1 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of HOOK1 in human colon, liver, lymph node and testis using HOOK1 Polyclonal Antibody (Product # PA5-53836) (A) shows similar protein distribution across tissues to an independent HOOK1 Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of HOOK1 in human endometrium using HOOK1 Polyclonal Antibody (Product # PA5-53836) shows no cytoplasmic positivity in glandular cells as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of HOOK1 in human testis using HOOK1 Polyclonal Antibody (Product # PA5-53836) shows weak to moderate positivity in spermatozoa and spermatids.
