10871-1-AP

antibody from Invitrogen Antibodies

Targeting: HOOK1 HK1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

10871-1-AP

Provider

Invitrogen Antibodies

Product name

HOOK1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: QVQDLHVKL SSESKRADTL AFEMKRLEEK HEALLKEKER LIEQRDTLKE TNEELRCSQV QQDHLNQTDA SATKSYENLA AEIMPVEYRE VFIRLQHENK MLRLQQEGSE NERIEELQEQ LEQKHRKMNE LETEQRLSKE RIRELQQQIE DLQKSLQEQG SKSEGESSSK LKQKLEAHME KLTEVHEELQ KKQELIEDLQ PDINQNVQKI NELEAALQKK DEDMKAMEER YKMYLEKARN VIKTLDPKLN PASAEIMLLR KQLAEKERRI EILESECKVA KFRDYEEKLI VSAWYNKSLA FQKLGMESRL VSGGGACSDT GACTPARSFL AQQRHITNTR RNLSVKVPAT TSD (377-728 aa encoded by BC011621)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.27 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 10871-1-AP (HOOK1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human brain tissue were subjected to SDS PAGE followed by western blot with 10871-1-AP (HOOK1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HEK-293 cells using 10871-1-AP (HOOK1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon cancer using 10871-1-AP (HOOK1 antibody) at dilution of 1:100 (under 10x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-HOOK1 (IP:10871-1-AP, 4ug; Detection:10871-1-AP 1:500) with mouse brain tissue lysate 4680ug.