Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [7]
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Validation data
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- Product number
- NBP1-81745 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-81745, RRID:AB_11036811
- Product name
- Rabbit Polyclonal HOOK1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human, mouse, rat HOOK1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Mouse Tafazzin Is Required for Male Germ Cell Meiosis and Spermatogenesis.
Cadalbert LC, Ghaffar FN, Stevenson D, Bryson S, Vaz FM, Gottlieb E, Strathdee D
PloS one 2015;10(6):e0131066
PloS one 2015;10(6):e0131066
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: HOOK1 Antibody [NBP1-81745] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HOOK1 Antibody [NBP1-81745] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HOOK1 Antibody [NBP1-81745] - Staining of human testis shows high expression.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HOOK1 Antibody [NBP1-81745] - Staining of human colon, liver, lymph node and testis using Anti-HOOK1 antibody NBP1-81745 (A) shows similar protein distribution across tissues to independent antibody NBP1-81744 (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HOOK1 Antibody [NBP1-81745] - Staining of human lymph node.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HOOK1 Antibody [NBP1-81745] - Staining of human liver.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HOOK1 Antibody [NBP1-81745] - Staining of human colon.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HOOK1 Antibody [NBP1-81745] - Analysis in human testis and endometrium tissues using antibody. Corresponding HOOK1 RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HOOK1 Antibody [NBP1-81745] - Staining of human endometrium shows no cytoplasmic positivity in glandular cells as expected.