11305-1-AP

antibody from Proteintech Group

Targeting: ECHS1 SCEH

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

11305-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#11305-1-AP, RRID:AB_2262166

Product name

ECHS1 antibody

Antibody type

Polyclonal

Description

KD/KO validated ECHS1 antibody (Cat. #11305-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB, ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Integrated spatial multi-omics delineates fatty acid degradation fuels malignant evolution at the tumour periphery in cervical squamous cell carcinoma.

Lin S, Luo Z, Li Y, Hu B, Qu M, Liu B, Cao C, Peng T, Xu M, Xu Y, Liu X, Wang X, Li L, Ding W, Xu Z, Li R, Zhao J, Zhang J, Wang L, Ren F, Wang C, Wu P
EBioMedicine 2026 May;127:106256

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Downregulated ECHS1 and HADH-mediated fatty acid β-oxidation contributes to mitochondrial dysfunction in salivary glands of Sjögren's syndrome.

Zhou M, Liu Y, He J, Li C, Wu B
Journal of translational autoimmunity 2026 Jun;12:100350

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Subacute exposure to thallium disrupts renal energy metabolism by impairing the function of subcellular organelles in aged mice of both sexes.

Yao H, Li L, Dai Q, Li D
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2025 Nov;205:115700

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ECHS1-NOX4 interaction suppresses rotenone-induced dopaminergic neurotoxicity through inhibition of mitochondrial ROS production.

Zhao Z, Wang R, Ge H, Hou L, Hatano T, Hattori N, Su H, Wang Q, Zhao J
Free radical biology & medicine 2025 May;232:56-71

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Acetylation-induced degradation of ECHS1 enhances BCAA accumulation and proliferation in KRAS-mutant colorectal cancer.

Li Z, Liu Z, Lin M, Pan H, Liu Y, Liu Y, Xie Y, Zhang J, Guan S, Li Y, Zhu M, Fang Y, Shen Z, Deng H
Journal of experimental & clinical cancer research : CR 2025 May 28;44(1):164

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Prenatal melatonin reprograms liver injury in male pups caused by maternal exposure to a high-fat diet and microplastics.

Chen YJ, Yu HR, Tsai CC, Tiao MM
Apoptosis : an international journal on programmed cell death 2025 Jun;30(5-6):1502-1514

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GRK5 as a Novel Therapeutic Target for Immune Evasion in Testicular Cancer: Insights from Multi-Omics Analysis and Immunotherapeutic Validation.

Xu C, Zhong Q, Yu N, Zhang X, Yang K, Liu H, Cai M, Zheng Y
Biomedicines 2025 Jul 21;13(7)

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Putative Role of Fatty Acid Metabolic Therapy Using Ketogenic Diet and HIF-1α Inhibition in Hepatocellular Carcinoma: Evidence from an In Vitro Study.

Kimura N, Kitajima Y, Higure K, Matsufuji S, Ikeda S, Furukawa S, Yukimoto K, Tanaka T, Noshiro H
International journal of molecular sciences 2025 Dec 5;26(24)

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Expression and clinical significance of ECHS1 in gastric cancer.

Lu T, Sun L, Fan Q, Yan J, Zhao D, Xu C, Dong F
Journal of Cancer 2024;15(2):418-427

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Identification of serum metabolites associated with aristolochic acid nephropathy severity and insights into the underlying mechanism.

Liu X, Peng Y, Wu S, Huang X, Gao L, Deng R, Lu J
Toxicology letters 2024 Oct;400:24-34

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Castration reshapes the liver by altering fatty acid composition and metabolism in male mice.

Yao H, Li D, Cao X, Han X, He J, Cheng D, Shang J, Song T, Zeng X
Biochemical and biophysical research communications 2024 Oct 1;727:150319

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Testosterone regulates thymic remodeling by altering metabolic reprogramming in male rats.

Li D, Yao H, Ren Y, Shang J, Han X, Cao X, Song T, Zeng X
General and comparative endocrinology 2024 Mar 1;348:114448

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Mitochondrial glycerol 3-phosphate dehydrogenase deficiency exacerbates lipotoxic cardiomyopathy.

Qu H, Liu X, Zhu J, He N, He Q, Zhang L, Wang Y, Gong X, Xiong X, Liu J, Wang C, Yang G, Yang Q, Luo G, Zhu Z, Zheng Y, Zheng H
iScience 2024 Jun 21;27(6):109796

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Valine and Inflammation Drive Epilepsy in a Mouse Model of ECHS1 Deficiency.

Eller MM, Zuberi AR, Fu X, Burgess SC, Lutz CM, Bailey RM
bioRxiv : the preprint server for biology 2024 Jun 13;

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YBX1 as a prognostic biomarker and potential therapeutic target in hepatocellular carcinoma: A comprehensive investigation through bioinformatics analysis and in vitro study.

Li Z, Lu W, Yin F, Huang A
Translational oncology 2024 Jul;45:101965

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Ginsenoside Rb1 regulates CPT1A deacetylation to inhibit intramuscular fat infiltration after rotator cuff tear.

Yin Y, Wang Z, Yang Y, Shen M, Hu H, Chen C, Zhou H, Li Z, Wu S
iScience 2024 Jul 19;27(7):110331

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MYC expression and fatty acid oxidation in EGFR-TKI acquired resistance.

Wang G, Li T, Wan Y, Li Q
Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy 2024 Jan;72:101019

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Hypoxia-induced downregulation of PGK1 crotonylation promotes tumorigenesis by coordinating glycolysis and the TCA cycle.

Guo Z, Zhang Y, Wang H, Liao L, Ma L, Zhao Y, Yang R, Li X, Niu J, Chu Q, Fu Y, Li B, Yang C
Nature communications 2024 Aug 12;15(1):6915

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Integrated bioinformatic analysis of mitochondrial metabolism-related genes in acute myeloid leukemia.

Tong X, Zhou F
Frontiers in immunology 2023;14:1120670

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Label-free quantitative proteomic analyses of mouse astrocytes provides insight into the host response mechanism at different developmental stages of Toxoplasma gondii.

Xie H, Sun H, Dong H, Dai L, Xu H, Zhang L, Wang Q, Zhang J, Zhao G, Xu C, Yin K
PLoS neglected tropical diseases 2023 Sep;17(9):e0011102

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Strategic validation of variants of uncertain significance in ECHS1 genetic testing.

Kishita Y, Sugiura A, Onuki T, Ebihara T, Matsuhashi T, Shimura M, Fushimi T, Ichino N, Nagatakidani Y, Nishihata H, Nitta KR, Yatsuka Y, Imai-Okazaki A, Wu Y, Osaka H, Ohtake A, Murayama K, Okazaki Y
Journal of medical genetics 2023 Oct;60(10):1006-1015

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CLPX regulates mitochondrial fatty acid β-oxidation in liver cells.

Suzuki K, Kubota Y, Kaneko K, Kamata CC, Furuyama K
The Journal of biological chemistry 2023 Oct;299(10):105210

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Long term N-acetylcysteine administration rescues liver steatosis via endoplasmic reticulum stress with unfolded protein response in mice.

Tsai CC, Chen YJ, Yu HR, Huang LT, Tain YL, Lin IC, Sheen JM, Wang PW, Tiao MM
Lipids in health and disease 2020 May 25;19(1):105

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Quantitative proteomics reveals molecular mechanism of gamabufotalin and its potential inhibition on Hsp90 in lung cancer.

Zhang L, Yu Z, Wang Y, Wang X, Zhang L, Wang C, Yue Q, Wang X, Deng S, Huo X, Tian X, Huang S, Zhang B, Ma X
Oncotarget 2016 Nov 22;7(47):76551-76564

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Opposing activity changes in AMP deaminase and AMP-activated protein kinase in the hibernating ground squirrel.

Lanaspa MA, Epperson LE, Li N, Cicerchi C, Garcia GE, Roncal-Jimenez CA, Trostel J, Jain S, Mant CT, Rivard CJ, Ishimoto T, Shimada M, Sanchez-Lozada LG, Nakagawa T, Jani A, Stenvinkel P, Martin SL, Johnson RJ
PloS one 2015;10(4):e0123509

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Cigarette smoke alters the proteomic profile of lung fibroblasts.

D'Anna C, Cigna D, Costanzo G, Bruno A, Ferraro M, Di Vincenzo S, Bianchi L, Bini L, Gjomarkaj M, Pace E
Molecular bioSystems 2015 Jun;11(6):1644-52

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Enoyl-coenzyme A hydratase short chain 1 silencing attenuates the proliferation of hepatocellular carcinoma by inhibiting epidermal growth factor signaling in vitro and in vivo.

Lin BY, Xiao CX, Zhao WX, Xiao L, Chen X, Li P, Wang XM
Molecular medicine reports 2015 Jul;12(1):1421-8

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Multifactorial comparative proteomic study of cytochrome P450 2E1 function in chronic alcohol administration.

Wang Y, Kou Y, Wang X, Cederbaum A, Wang R
PloS one 2014;9(3):e92504

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Novel proteomic biomarker panel for prediction of aggressive metastatic hepatocellular carcinoma relapse in surgically resectable patients.

Tan GS, Lim KH, Tan HT, Khoo ML, Tan SH, Toh HC, Ching Ming Chung M
Journal of proteome research 2014 Nov 7;13(11):4833-46

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The day/night proteome in the murine heart.

Podobed P, Pyle WG, Ackloo S, Alibhai FJ, Tsimakouridze EV, Ratcliffe WF, Mackay A, Simpson J, Wright DC, Kirby GM, Young ME, Martino TA
American journal of physiology. Regulatory, integrative and comparative physiology 2014 Jul 15;307(2):R121-37

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Small hepatitis B surface antigen interacts with and modulates enoyl-coenzyme A hydratase expression in hepatoma cells.

Gong X, Zhu Y, Dong J, Chen J, You J, Zheng Q, Rao Z, Mao Q, Jiang J
Archives of virology 2013 May;158(5):1065-70

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Counteracting roles of AMP deaminase and AMP kinase in the development of fatty liver.

Lanaspa MA, Cicerchi C, Garcia G, Li N, Roncal-Jimenez CA, Rivard CJ, Hunter B, Andrés-Hernando A, Ishimoto T, Sánchez-Lozada LG, Thomas J, Hodges RS, Mant CT, Johnson RJ
PloS one 2012;7(11):e48801

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Uric acid-induced endothelial dysfunction is associated with mitochondrial alterations and decreased intracellular ATP concentrations.

Sánchez-Lozada LG, Lanaspa MA, Cristóbal-García M, García-Arroyo F, Soto V, Cruz-Robles D, Nakagawa T, Yu MA, Kang DH, Johnson RJ
Nephron. Experimental nephrology 2012;121(3-4):e71-8

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Sucrose induces fatty liver and pancreatic inflammation in male breeder rats independent of excess energy intake.

Roncal-Jimenez CA, Lanaspa MA, Rivard CJ, Nakagawa T, Sanchez-Lozada LG, Jalal D, Andres-Hernando A, Tanabe K, Madero M, Li N, Cicerchi C, Mc Fann K, Sautin YY, Johnson RJ
Metabolism: clinical and experimental 2011 Sep;60(9):1259-70

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The Krebs cycle and mitochondrial mass are early victims of endothelial dysfunction: proteomic approach.

Addabbo F, Ratliff B, Park HC, Kuo MC, Ungvari Z, Csiszar A, Krasnikov B, Sodhi K, Zhang F, Nasjletti A, Goligorsky MS
The American journal of pathology 2009 Jan;174(1):34-43

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One-hit effects in cancer: altered proteome of morphologically normal colon crypts in familial adenomatous polyposis.

Yeung AT, Patel BB, Li XM, Seeholzer SH, Coudry RA, Cooper HS, Bellacosa A, Boman BM, Zhang T, Litwin S, Ross EA, Conrad P, Crowell JA, Kopelovich L, Knudson A
Cancer research 2008 Sep 15;68(18):7579-86

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Proteomic analysis of left ventricular remodeling in an experimental model of heart failure.

Cieniewski-Bernard C, Mulder P, Henry JP, Drobecq H, Dubois E, Pottiez G, Thuillez C, Amouyel P, Richard V, Pinet F
Journal of proteome research 2008 Nov;7(11):5004-16

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

human liver tissue were subjected to SDS PAGE followed by western blot with 11305-1-AP(ECHS1 antibody) at dilution of 1:1200

Sample type

tissue

Immunoprecipitation

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

IP Result of anti-ECHS1 (IP:11305-1-AP, 3ug; Detection:11305-1-AP 1:500) with PC-3 cells lysate 3000ug.

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The ECHS1 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human ECHS1. This antibody recognizes human,mouse,rat antigen. The ECHS1 antibody has been validated for the following applications: ELISA, WB, IHC, IP analysis.

Appendix

11305-1-AP.pdf

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