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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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- Product number
- GTX114375 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX114375, RRID:AB_10620333
- Product name
- ECHS1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ECHS1 antibody detects ECHS1 protein by Western blot analysis.A. 30 ?g NT2D1 whole cell lysate/extract B. 30 ug PC-3 whole cell lysate/extractC. 30 ug U87-MG whole cell lysate/extractD. 30 ug SK-N-SH whole cell lysate/extract12 % SDS-PAGEECHS1 antibody (GTX114375) dilution: 1:3000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ECHS1 antibody detects ECHS1 protein by Western blot analysis.A. 30 ?g Neuro2A whole cell lysate/extract B. 30 ?g GL261 whole cell lysate/extractC. 30 ?g C8D30 whole cell lysate/extract12 % SDS-PAGEECHS1 antibody (GTX114375) dilution: 1:1000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed HeLa, using ECHS1(GTX114375) antibody at 1:200 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ECHS1 antibody detects ECHS1 protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: ECHS1 protein stained by ECHS1 antibody (GTX114375) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of ECHS1 protein from 293T whole cell extracts using 5 £gg of ECHS1 antibody (GTX114375).Western blot analysis was performed using ECHS1 antibody (GTX114375).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ECHS1 antibody detects ECHS1 protein at mitochondria on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. ECHS1 antibody (GTX114375) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ECHS1 antibody detects ECHS1 protein at mitochondria on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. ECHS1 antibody (GTX114375) dilution: 1:500.
