PA5-30013

antibody from Invitrogen Antibodies

Targeting: ECHS1 SCEH

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-30013

Provider

Invitrogen Antibodies

Product name

ECHS1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: NT2D1, PC-3, U87-MG, SK-N-SH, Neuro2A, GL261, C8D30.

Concentration

0.8 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ECHS1 Polyclonal Antibody detects ECHS1 protein by Western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract. B. 30 µg GL261 whole cell lysate/extract. C. 30 µg C8D30 whole cell lysate/extract.12 % SDS-PAGE. ECHS1 Polyclonal Antibody (Product # PA5-30013) dilution: 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ECHS1 Polyclonal Antibody detects ECHS1 protein by Western blot analysis. A. 30 µg NT2D1 whole cell lysate/extract. B. 30 µg PC-3 whole cell lysate/extract. C. 30 µg U87-MG whole cell lysate/extract. D. 30 µg SK-N-SH whole cell lysate/extract.12 % SDS-PAGE. ECHS1 Polyclonal Antibody (Product # PA5-30013) dilution: 1:3,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of ECHS1 was performed in HeLa cells fixed in ice-cold MeOH for 5 min. Green: ECHS1 Polyclonal Antibody (Product # PA5-30013) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ECHS1 Polyclonal Antibody detects ECHS1 protein at mitochondria on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. ECHS1 Polyclonal Antibody (Product # PA5-30013) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ECHS1 Polyclonal Antibody detects ECHS1 protein at mitochondria on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. ECHS1 Polyclonal Antibody (Product # PA5-30013) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of ECHS1 was performed in 293T whole cell extracts using 5 µg of ECHS1 Polyclonal Antibody (Product # PA5-30013). Samples were transferred to a membrane and probed with ECHS1 Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.