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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [4]
- Immunoprecipitation [1]
- Immunohistochemistry [4]
- Other assay [1]
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- Product number
- PA5-30014 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ECHS1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2, mouse brain. Predicted reactivity: Human (99%), Mouse (87%), Rat (85%), Pig (81%), Bovine (82%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.01 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of ECHS1 was performed in A431 cells fixed in 2% paraformaldehyde/culture medium at RT for 30 min. Green: ECHS1 Polyclonal Antibody (Product # PA5-30014) diluted at 1:200. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of ECHS1 was performed in HeLa cells fixed in ice-cold MeOH for 5 min. Green: ECHS1 Polyclonal Antibody (Product # PA5-30014) diluted at 1:500. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of ECHS1 was performed in A431 cells fixed in 2% paraformaldehyde/culture medium at RT for 30 min. Green: ECHS1 Polyclonal Antibody (Product # PA5-30014) diluted at 1:200. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of ECHS1 was performed in HeLa cells fixed in ice-cold MeOH for 5 min. Green: ECHS1 Polyclonal Antibody (Product # PA5-30014) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of ECHS1 was performed in 293T whole cell extracts using 5 µg of ECHS1 Polyclonal Antibody (Product # PA5-30014). Samples were transferred to a membrane and probed with ECHS1 Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ECHS1 Polyclonal Antibody detects ECHS1 protein at mitochondria on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse ovary. ECHS1 Polyclonal Antibody (Product # PA5-30014) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ECHS1 Polyclonal Antibody detects ECHS1 protein at mitochondria on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. ECHS1 Polyclonal Antibody (Product # PA5-30014) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ECHS1 Polyclonal Antibody detects ECHS1 protein at mitochondria on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. ECHS1 Polyclonal Antibody (Product # PA5-30014) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ECHS1 Polyclonal Antibody detects ECHS1 protein at mitochondria on mouse ovary by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. ECHS1 Polyclonal Antibody (Product # PA5-30014) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of ECHS1 was performed in 293T whole cell extracts using 5 µg of ECHS1 Polyclonal Antibody (Product # PA5-30014). Samples were transferred to a membrane and probed with ECHS1 Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.
