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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [4]
- Other assay [1]
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- Product number
- PA5-30014 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ECHS1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2, mouse brain.
- Concentration
- 1.01 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ECHS1 Polyclonal Antibody (Product # PA5-30014). Sample (50 µg of whole cell lysate). Lane A: mouse brain. 12% SDS PAGE. ECHS1 Polyclonal Antibody (Product # PA5-30014) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ECHS1 Polyclonal Antibody detects ECHS1 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with ECHS1 Polyclonal Antibody (Product # PA5-30014) diluted by 1:5,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of ECHS1 was performed in A431 cells fixed in 2% paraformaldehyde/culture medium at RT for 30 min. Green: ECHS1 Polyclonal Antibody (Product # PA5-30014) diluted at 1:200. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of ECHS1 was performed in HeLa cells fixed in ice-cold MeOH for 5 min. Green: ECHS1 Polyclonal Antibody (Product # PA5-30014) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ECHS1 Polyclonal Antibody detects ECHS1 protein at mitochondria on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. ECHS1 Polyclonal Antibody (Product # PA5-30014) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ECHS1 Polyclonal Antibody detects ECHS1 protein at mitochondria on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. ECHS1 Polyclonal Antibody (Product # PA5-30014) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ECHS1 Polyclonal Antibody detects ECHS1 protein at mitochondria on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse ovary. ECHS1 Polyclonal Antibody (Product # PA5-30014) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ECHS1 Polyclonal Antibody detects ECHS1 protein at mitochondria on mouse ovary by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. ECHS1 Polyclonal Antibody (Product # PA5-30014) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of ECHS1 was performed in 293T whole cell extracts using 5 µg of ECHS1 Polyclonal Antibody (Product # PA5-30014). Samples were transferred to a membrane and probed with ECHS1 Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.
