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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
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- References [1]
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- Western blot [4]
- Immunoprecipitation [1]
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- Product number
- NBP1-30912 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-30912, RRID:AB_2117950
- Product name
- Rabbit Polyclonal HES-1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Osteoblast Hypoxia-Inducible Factor-1α Pathway Activation Restrains Osteoclastogenesis via the Interleukin-33-MicroRNA-34a-Notch1 Pathway.
Kang H, Yang K, Xiao L, Guo L, Guo C, Yan Y, Qi J, Wang F, Ryffel B, Li C, Deng L
Frontiers in immunology 2017;8:1312
Frontiers in immunology 2017;8:1312
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HES-1 Antibody [NBP1-30912] - PC-12 whole cell extract.12 % SDS-PAGE HES1 antibody [N1], N-term dilution: 1:1000
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HES-1 Antibody [NBP1-30912] - Sample(30 ug whole cell lysate)A:A431 12% SDS PAGE, antibody diluted at 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HES-1 Antibody [NBP1-30912] - Mouse kidney extract.12 % SDS-PAGE HES1 antibody [N1], N-term dilution: 1:1000
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HES-1 Antibody [NBP1-30912] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with HES1 antibody [N1], N-term (NBP1-30912). The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: HES-1 Antibody [NBP1-30912] - A431 whole cell lysate/extract A : Control with 2.5 ug of pre-immune rabbit IgG B : Immunoprecipitation of HES1 by 2.5 ug of HES1 antibody 12% SDS-PAGE The immunoprecipitated HES1 protein was detected by HES1 antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.
