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LearnA300-541A
antibody from Invitrogen Antibodies
Targeting: EP400
CAGH32, DKFZP434I225, KIAA1498, KIAA1818, P400, TNRC12
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Other assay [1]
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- Product number
- A300-541A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- p400 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.20 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of mouse p400 by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-p400 antibody (Product # A300-541A; lot A300-541A-2) used for WB at 0.4 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human p400 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, K-562, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-p400 antibody (Product # A300-541A; lot A300-541A-2) used for WB at 0.4 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of mouse p400 by western blot. Samples: Whole cell lysate from CT26.WT (15 and 50 µg) cells. Antibodies: Affinity purified rabbit anti-p400 antibody A300-541A (lot A300-541A-1) used for WB at 0.2 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human p400 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 1/4 of IP reaction loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-p400 antibody BL1879 (Cat. No. A300-541A) used at 0.04 µg/ml for WB (A and B) and at 3 µg/mg lysate for IP. immunoprecipitation of p400 was also accomplished, albeit less efficiently, using rabbit anti-p400 antibody BL1878, which is to an upstream epitope on p400. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human p400 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-p400 antibody (Product # A300-541A; lot A300-541A-2) used for IP at 6 µg per reaction. p400 was also immunoprecipitated by a previous lot of this antibody (lot A300-541A-1) and rabbit anti-p400 antibody BL1876. For blotting immunoprecipitated p400, Product # A300-541A was used at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
