20710-1-AP

antibody from Proteintech Group

Targeting: ACSL3 ACS3, FACL3, PRO2194

Provider product page for 20710-1-AP

Western blot

ELISA

Immunocytochemistry

Antibody data

Antibody Data
Antigen structure
References [35]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]

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Product number: 20710-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#20710-1-AP, RRID:AB_10697670
Product name: ACSL3 antibody
Antibody type: Polyclonal
Description: KD/KO validated ACSL3 antibody (Cat. #20710-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: IF, WB, ELISA.
Reactivity: Human, Mouse
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

ACSL3 protein structure - 20710-1-AP shown in red.

Submitted references Vitamin A and its analogues modulate MUFAs metabolism to improve ferroptosis and aging by direct targeting of ACSL3.

Luo N, Xiao Y, Zhai Y, Li J, Lv L, Yin H, Lin F, Wan B, Zhang K, Hu J, Liu J, Dang Y, He Y, Zhao Y, Zhang Z, Nie S, Yuan HX
Acta pharmaceutica Sinica. B 2026 Mar;16(3):1510-1529

Methotrexate-triggered ferroptosis suppresses oral cancer progression by phosphorylated KEAP1-mediated NRF2 degradation to inhibit SLC7A11/GPX4 signaling pathway.

Yu C, Zhang T, Yuan J, Su Y, Zhang H, Xu L, Li X, Cui J, Xu R, Zhou Y, Huang H, Zhou X, Zhou Y, Zhang E
Cancer cell international 2026 Jan 6;26(1):64

The UFL1-AKT positive feedback loop promotes breast cancer progression by enhancing lipid synthesis.

Meng F, Du Y, Liang J, Li H, Wang J, Tang K, Kong R, Sun H, Yin T, Qin J, Yang X, Shan C, Liu M, Yang G, Zhang J, Wang Y, Zhou J, Chen Y
Nature communications 2026 Jan 20;17(1):614

NT5DC2 inhibits ferroptosis by stabilizing ACSL3 in bladder cancer.

Niu S, Yang P, Yao Y, Tang X, Yang J, Zhang F, Chen K, Jiang C, Zhou Y, Bai W, Li L, Zhou Y, Lv XB
Cell death discovery 2026 Apr 14;12(1)

Multidimensional analysis reveals the potential of ACSL3 as a cancer biomarker: from pan-cancer exploration to functional validation in hepatocellular carcinoma.

Niu Q, Mou Y, Yao Y, Dong H, Wang K, Zeng Z, Tao Y, Gong X, Li H
Clinical and experimental medicine 2025 Oct 31;25(1):351

Co-administration of berberine and evodiamine: Mitigating evodiamine-induced hepatotoxicity and potentiating colitis treatment.

Tao X, Gao Y, Jin Z, Lu S, Li Z, Guo S, Fan Q, Li J, Qiao C, Lei H, Zhang Z, Zhao C, Wu J
Phytomedicine : international journal of phytotherapy and phytopharmacology 2025 Nov;147:157185

Integration of transcriptomics and metabolomics to reveal crizotinib-induced liver injury in mice.

Chen H, Liu H, Wei J, Liu R, Tian X
European journal of pharmacology 2025 Nov 5;1006:178096

Sec14L6 is a phosphoinositide transporter that regulates phosphoinositide homeostasis and biogenesis of lipid droplets.

Zhou T, Xiong J, Hu X, Du Y, Shi A, Chang W, Qin J, Deng L, Ji WK
Nature communications 2025 Nov 26;16(1):10518

Targeting YBX1-m5C mediates RNF115 mRNA circularisation and translation to enhance vulnerability of ferroptosis in hepatocellular carcinoma.

Li O, An K, Wang H, Li X, Wang Y, Huang L, Du Y, Qin N, Dong J, Wei J, Sun R, Shi Y, Guo Y, Sun X, Yang Y, Yang YG, Kan Q, Tian X
Clinical and translational medicine 2025 Mar;15(3):e70270

Methyltransferase-like 7B participates in bladder cancer via ACSL3 m(6)A modification in a ferroptosis manner.

He J, Dong C, Song X, Qiu Z, Zhang H, Jiang Y, Liu T, Man X
Biology direct 2025 Jan 20;20(1):9

Attenuated growth factor signaling during cell death initiation sensitizes membranes towards peroxidation.

Gollowitzer A, Pein H, Rao Z, Waltl L, Bereuter L, Loeser K, Meyer T, Jafari V, Witt F, Winkler R, Su F, Große S, Thürmer M, Grander J, Hotze M, Harder S, Espada L, Magnutzki A, Gstir R, Weinigel C, Rummler S, Bonn G, Pachmayr J, Ermolaeva M, Harayama T, Schlüter H, Kosan C, Heller R, Thedieck K, Schmitt M, Shimizu T, Popp J, Shindou H, Kwiatkowski M, Koeberle A
Nature communications 2025 Feb 25;16(1):1774

Endosomal trafficking participates in lipid droplet catabolism to maintain lipid homeostasis.

Peng W, Chen S, Ma J, Wei W, Lin N, Xing J, Guo W, Li H, Zhang L, Chan K, Yen A, Zhu G, Yue J
Nature communications 2025 Feb 24;16(1):1917

TMEM120A maintains adipose tissue lipid homeostasis through ER CoA channeling.

Cho YK, Lee J, Jeong YL, Shim M, Mai XL, Seomoon C, Jung CW, Choi YH, Namgoong S, Jung YS, Kwon SW, Lee J, Seong JK, Park S, Mottillo EP, Granneman JG, Lee DK, Kim JK, Lee YH
Nature communications 2025 Dec 21;17(1):1113

A novel SOX6 + melanoma cell subtype promotes early microsatellite invasion in Asian acral melanoma through fatty acid transport disorder.

Lv C, Chen K, Wang T, Jiang J, Hu G, Gu J, Liu T, Wang S, Dai H, Wang Y
Journal of experimental & clinical cancer research : CR 2025 Aug 27;44(1):254

Resveratrol induces ferroptosis in triple-negative breast cancer through NEDD4L-mediated GPX4 ubiquitination and degradation.

Zhang E, Wang Y, Zhang H, Li X, Su Y, Cui J, Xu R, Mao X, Sang M, Lin Z, Zhou X
Free radical biology & medicine 2025 Aug 1;235:231-247

Role of ferroptosis mediated by abnormal membrane structure in DEHP-induced reproductive injury.

Deng W, Zhao J, Wang X, Li D, Wang M, Zheng X, Wang R, Guo Q, Zhao P, Yan H, Shen L, Long C, Wei G, Wu S
Free radical biology & medicine 2025 Aug 1;235:150-161

ACSL3 is a promising therapeutic target for alleviating anxiety and depression in Alzheimer's disease.

Wu CY, Zhang Y, Howard P, Huang F, Lee RH
GeroScience 2025 Apr;47(2):2383-2397

p38 mediated ACSL4 phosphorylation drives stress-induced esophageal squamous cell carcinoma growth through Src myristoylation.

Yuan Q, Shi Y, Wang J, Xie Y, Li X, Zhao J, Jiang Y, Qiao Y, Guo Y, Zhang C, Lu J, Zhao T, Dong Z, Li P, Dong Z, Liu K
Nature communications 2025 Apr 7;16(1):3319

Exploring the ATG9A interactome uncovers interaction with VPS13A.

van Vliet AR, Jefferies HBJ, Faull PA, Chadwick J, Ibrahim F, Skehel MJ, Tooze SA
Journal of cell science 2024 Feb 15;137(4)

Crizotinib and its enantiomer suppress ferroptosis by decreasing PE-O-PUFA content.

Cen SY, Lin F, Li X, Hu Y, Liu JP, Xue Z, Gao Y, Sun YP, Zhu S, Dang Y, Zhao Y, Yuan HX
Cell death discovery 2024 Aug 12;10(1):360

Integrative analysis of network pharmacology and proteomics reveal the protective effect of Xiaoqinglong Decotion on neutrophilic asthma.

Liao G, Yan Q, Zhang M, Zhang X, Yang J, Huang H, Liu X, Jiang Y, Gong J, Zhan S, Li D, Huang X
Journal of ethnopharmacology 2024 Aug 10;330:118102

Anti-apoptotic MCL-1 promotes long-chain fatty acid oxidation through interaction with ACSL1.

Wright T, Turnis ME, Grace CR, Li X, Brakefield LA, Wang YD, Xu H, Kaminska E, Climer LK, Mukiza TO, Chang CL, Moldoveanu T, Opferman JT
Molecular cell 2024 Apr 4;84(7):1338-1353.e8

Blockage of EGFR/AKT and mevalonate pathways synergize the antitumor effect of temozolomide by reprogramming energy metabolism in glioblastoma.

Cui X, Zhao J, Li G, Yang C, Yang S, Zhan Q, Zhou J, Wang Y, Xiao M, Hong B, Yi K, Tong F, Tan Y, Wang H, Wang Q, Jiang T, Fang C, Kang C
Cancer communications (London, England) 2023 Dec;43(12):1326-1353

One amino acid drives the lipid droplet targeting sequence of a new noncoding RNA-encoded protein to mitochondrion.

Yuan Q, Zheng K, Huang T, Zhi Z, Xu Y, Cui L, Liu P, Zhang S
Proteomics 2023 Aug;23(15):e2200301

Construction and validation of a novel ferroptosis-related prognostic signature for lung adenocarcinoma.

Yang L, Fan X, Zhou C, Wang Z, Cui Z, Wu X, Xu Z, Yang J, Zhang X
Translational lung cancer research 2023 Aug 30;12(8):1766-1781

ACSL3 is a potential prognostic biomarker for immune infiltration in clear cell renal cell carcinoma.

Zhang C, Hu H, Huang R, Huang G, Xi X
Frontiers in surgery 2022;9:909854

NAT10: An RNA cytidine transferase regulates fatty acid metabolism in cancer cells.

Dalhat MH, Mohammed MRS, Alkhatabi HA, Rehan M, Ahmad A, Choudhry H, Khan MI
Clinical and translational medicine 2022 Sep;12(9):e1045

Inhibition of Fatty Acid Translocase (FAT/CD36) Palmitoylation Enhances Hepatic Fatty Acid β-Oxidation by Increasing Its Localization to Mitochondria and Interaction with Long-Chain Acyl-CoA Synthetase 1.

Zeng S, Wu F, Chen M, Li Y, You M, Zhang Y, Yang P, Wei L, Ruan XZ, Zhao L, Chen Y
Antioxidants & redox signaling 2022 Jun;36(16-18):1081-1100

Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.

Chu H, Zhao Q, Liu J, Yang K, Wang Y, Liu J, Zhang K, Zhao B, He H, Zheng Y, Zhong S, Liang Z, Zhang L, Zhang Y
Analytical chemistry 2022 Jan 18;94(2):758-767

N(6)-methyladenosine (m(6)A) in 18S rRNA promotes fatty acid metabolism and oncogenic transformation.

Peng H, Chen B, Wei W, Guo S, Han H, Yang C, Ma J, Wang L, Peng S, Kuang M, Lin S
Nature metabolism 2022 Aug;4(8):1041-1054

Changes of lipid profiles in human umbilical vein endothelial cells exposed to zirconia nanoparticles with or without the presence of free fatty acids.

Cheng X, Guo H, Xian Y, Xie X
Journal of applied toxicology : JAT 2021 May;41(5):765-774

Rab18 binds PLIN2 and ACSL3 to mediate lipid droplet dynamics.

Deng Y, Zhou C, Mirza AH, Bamigbade AT, Zhang S, Xu S, Liu P
Biochimica et biophysica acta. Molecular and cell biology of lipids 2021 Jul;1866(7):158923

Transcriptomic analysis suggested the involvement of impaired lipid droplet biogenesis in graphene oxide-induced cytotoxicity in human umbilical vein endothelial cells.

Luo Y, Wang X, Cao Y
Chemico-biological interactions 2021 Jan 5;333:109325

Integrated Metabolomics and Lipidomics Analyses Reveal Metabolic Reprogramming in Human Glioma with IDH1 Mutation.

Zhou L, Wang Z, Hu C, Zhang C, Kovatcheva-Datchary P, Yu D, Liu S, Ren F, Wang X, Li Y, Hou X, Piao H, Lu X, Zhang Y, Xu G
Journal of proteome research 2019 Mar 1;18(3):960-969

Morphologically and Functionally Distinct Lipid Droplet Subpopulations.

Zhang S, Wang Y, Cui L, Deng Y, Xu S, Yu J, Cichello S, Serrero G, Ying Y, Liu P
Scientific reports 2016 Jul 8;6:29539

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20710-1-AP(ACSL3 antibody) at dilution of 1:300
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of HepG2 cells, using ACSL3 antibody 20710-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line