PA5-31227

antibody from Invitrogen Antibodies

Targeting: OLA1 GTPBP9, PTD004

Antibody data

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Validation data

Product number: PA5-31227 - Provider product page
Provider: Invitrogen Antibodies
Product name: GTPBP9 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Recommended positive controls: 293T, A431, HeLa, HepG2, mouse testis, rat testis. Predicted reactivity: Mouse (99%), Rat (99%), Zebrafish (89%), Xenopus laevis (85%), Chicken (90%), Rhesus Monkey (100%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 0.46 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

OLA1 protein structure - PA5-31227 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of GTPBP9 using 30 µg of HepG2 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a GTPBP9 polyclonal antibody (Product # PA5-31227) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of GTPBP9 using 50 µg of mouse brain lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a GTPBP9 polyclonal antibody (Product # PA5-31227) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of GTPBP9 was performed by separating 50 µg of mouse tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a GTPBP9 Polyclonal Antibody (Product # PA5-31227) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of GTPBP9 was performed by separating 50 µg of rat tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a GTPBP9 Polyclonal Antibody (Product # PA5-31227) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of GTPBP9 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a GTPBP9 Polyclonal Antibody (Product # PA5-31227) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.