Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-31228 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GTPBP9 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2, mouse testis, rat testis. Predicted reactivity: Mouse (99%), Rat (97%), Zebrafish (83%), Xenopus laevis (85%), Chicken (94%), Rhesus Monkey (100%), Bovine (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.53 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GTPBP9 using 30 µg of HepG2 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a GTPBP9 polyclonal antibody (Product # PA5-31228) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GTPBP9 using 50 µg of mouse brain lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a GTPBP9 polyclonal antibody (Product # PA5-31228) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GTPBP9 was performed by separating 50 µg of mouse tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a GTPBP9 Polyclonal Antibody (Product # PA5-31228) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GTPBP9 was performed by separating 50 µg of rat tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a GTPBP9 Polyclonal Antibody (Product # PA5-31228) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of GTPBP9 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a GTPBP9 Polyclonal Antibody (Product # PA5-31228) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using GTPBP9 (Product # PA5-31228) antibody at 1:250 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.