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Western blot
Immunocytochemistry
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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Validation data
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- Product number
- HPA024018 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA024018, RRID:AB_1849891
- Product name
- Anti-GOPC
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human GOPC, Gene description: golgi-associated PDZ and coiled-coil motif containing, Alternative Gene Names: CAL, dJ94G16.2, FIG, GOPC1, PIST, Validated applications: WB, IHC, ICC, Uniprot ID: Q9HD26, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.05 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references
Tex46 knockout male mice are sterile secondary to sperm head malformations and failure to penetrate through the zona pellucida
PDCL2 is essential for sperm acrosome formation and male fertility in mice
Fujihara Y, Miyata H, Abbasi F, Larasati T, Nozawa K, Yu Z, Ikawa M, Matzuk M, Yortsos Y
PNAS Nexus 2024;3(3)
PNAS Nexus 2024;3(3)
PDCL2 is essential for sperm acrosome formation and male fertility in mice
Fujihara Y, Kobayashi K, Abbasi F, Endo T, Yu Z, Ikawa M, Matzuk M
Andrology 2022;11(5):789-798
Andrology 2022;11(5):789-798
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Enhanced validation
Enhanced validation
- Submitted by
- klas2
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot of cell lysate from U-2 OS cells transfected with either siRNA targeting GOPC or control siRNA. Lane 1: Marker (250, 130, 95, 72, 55, 36, 28, 17, 10) Lane 2: Cell lysate from U-2OS cells transfected with siRNA targeting GOPC Lane 3: N/A Lane 4: Cell lysate from U-2OS cells transfected with control siRNA Right image, lane 1-4: loading control
- Sample type
- U-2 OS
- Primary Ab dilution
- 1:58
- Conjugate
- Horseradish Peroxidase
- Secondary Ab
- Secondary Ab
- Secondary Ab dilution
- 1:3000
- Knockdown/Genetic Approaches Application
- Western blot
Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1, using Anti-GOPC antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
- Sample type
- Human
- Protocol
- Protocol
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus.
- Sample type
- Human
