Explore
Validate
Learn
Western blot
Immunocytochemistry
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [3]
- Flow cytometry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA5-34771 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PIST Recombinant Rabbit Monoclonal Antibody (JG79-35)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: SiHa, HepG2, MCF-7, SKOV-3, human liver cancer tissue, human colon tissue, human kidney tissue, mouse brain tissue.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- JG79-35
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles, store in dark
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of PIST in SKOV-3 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with PIST monoclonal antibody (Product # MA5-34771), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of PIST in MCF-7 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with PIST monoclonal antibody (Product # MA5-34771), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of PIST in HepG2 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with PIST monoclonal antibody (Product # MA5-34771), followed by DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of PIST in MCF-7 cells (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Samples were incubated with PIST monoclonal antibody (Product # MA5-34771) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of PIST in MCF-7 cells (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Samples were incubated with PIST monoclonal antibody (Product # MA5-34771) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.
