GTX31230

antibody from GeneTex

Targeting: NCR1 CD335, LY94, NK-p46, NKP46

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX31230

Provider

GeneTex

Product name

NKp46 antibody [AKS1]

Antibody type

Monoclonal

Reactivity

Bovine

Host

Mouse

Flow cytometry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Staining of bovine peripheral blood lymphocytes with Mouse anti Bovine CD335 followed by Goat anti Mouse IgG (H/L):FITC. A small positive peak representing NK cells can be identified

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Published customer image: Mouse anti bovine CD335 antibody used for the evaluation of NKp46 expression on bovine peripheral blood mononuclear cells by flow cytometry Image caption: Oenothein B Primes bovine CD335+ cells to respond to IL-18. (A) Bovine PBMCs (105 cells/well) were depleted of CD335+ cells and treated with 20 μg/ml oenothein B or X-VIVO medium alone for 24 hrs. Cells were then washed and treated with 10 ng/ml rhu IL-18 or medium alone for 18 hrs. After incubation, IFN levels in the supernatant fluids were measured by ELISA. The data are expressed as mean +/- SEM of three independent experiments comparing depleted PBMCs to un-depleted controls tested concurrently. All samples were tested in duplicate or triplicate. Statistical significance was measured by Two-way ANOVA with Bonferroni post-test. *p From: Ramstead AG, Schepetkin IA, Quinn MT, Jutila MA (2012) Oenothein B, a Cyclic Dimeric Ellagitannin Isolated from Epilobium angustifolium, Enhances IFN Production by Lymphocytes. PLoS ONE 7(11): e50546 .

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Published customer image: Mouse anti bovine CD335 antibody, clone AKS1 used for the isolation by immunomagnetic separation and evaluation of CD335 expression by flow cytometry of NKp46 expression on bovine peripheral blood mononuclear cells. Image caption: CD2 distribution on bovine NK cells in peripheral blood and during cell culture. (A) CD2 expression by PBMCs from healthy cattle, and in NKp46-selected cells cultured for the indicated time in the presence of 100U/ml rbIL-2. Results were obtained by two-colour flow cytometry, gating for viable lymphocytes. Data shown are from one animal representative of 19. (B) Monitoring of cell divisions of CD2- and CD2+ NK cell populations during three and six days of rbIL-2 (100U/ml) stimulation. NKp46-selected cells were labelled with CFSE the day after isolation and CFSE intensity (filled histograms) measured in flow cytometry on the indicated days, gating for CD2- or CD2+ viable cells. Solid line open histograms show non-dividing NK cells, cultured in the presence of 1U/ml rbIL-2, while broken line open histograms show unlabeled stimulated NK cells. Data shown are from one animal representative of five. (C) Stability of CD2 expression in NK cell subsets cultured separately. NK cells separated with immunomagnetic beads for absence or presence of CD2 were cultured separately for eleven days in the presence of IL-2, monitoring CD2 expression at three different time points (filled histograms). Day 1 corresponds to the day of separation, 24 h after primary NK cell isolation. Open histograms show secondary mAb controls. Data shown are from one animal representative of eight. From: Boysen P, Olsen I, Berg I, Kulberg S, Johansen GM, Storset AK. Bovine CD2-/NKp46+ cells are fully functional natural killer cells with a high activation status. BMC Immunol. 2006 Apr 27;7:10 .

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Published customer image: Mouse anti bovine CD335 antibody used for the evaluation of NKp46 expression on bovine peripheral blood mononuclear cells by flow cytometry Image caption: Oenothein B induces IL-2Ra or CD69 on bovine and human NK cells. (A) Bovine PBMCs (105 cells/well) were treated with 20 μg/ml oenothein B in X-VIVO medium for 24 hrs, and IL-2Ra expression on NK cells was measured by multi-color flow cytometry. Representative examples of two-color flow cytometry plots comparing IL-2Ra staining on oenothein B-treated and untreated bovine NK cells (CD335+) from each animal are shown. (B) Human PBMCs (105 cells/well) were treated with 40 μg/ml oenothein B in cRPMI medium for 48 hrs. CD69 expression on NK cells was then measured by flow cytometry. Representative examples of two-color flow cytometry plots comparing CD69 staining on oenothein B-treated and untreated human NK cells from each donor are shown. From: Ramstead AG, Schepetkin IA, Quinn MT, Jutila MA (2012) Oenothein B, a Cyclic Dimeric Ellagitannin Isolated from Epilobium angustifolium, Enhances IFN Production by Lymphocytes. PLoS ONE 7(11): e50546 .