Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 17-3359-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD335 (NKp46) Monoclonal Antibody (9E2), APC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This 9E2 monoclonal antibody reacts with human CD335, which is also known as NKp46. Expressed on NK cells, CD335 is a member of the natural cytotoxicity receptor family that also includes NKp30 and NKp44. This type I transmembrane glycoprotein is involved in activating and expanding resting NK cells following interaction with dendritic cells to eliminate tumor and virus-infected (e.g., influenza) cells. CD335 also interacts with pancreatic beta cells during the development of type I diabetes. The 9E2 antibody has been reported to induce cytotoxicity. Applications Reported: This 9E2 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 9E2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human periheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 9E2
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references The number and cytotoxicity and the expression of cytotoxicity-related molecules in peripheral natural killer (NK) cells do not predict the repeated implantation failure (RIF) for the in vitro fertilization patients.
Induction of the Lytic Cycle Sensitizes Epstein-Barr Virus-Infected B Cells to NK Cell Killing That Is Counteracted by Virus-Mediated NK Cell Evasion Mechanisms in the Late Lytic Cycle.
Plasmacytoid dendritic cells and C1q differentially regulate inflammatory gene induction by lupus immune complexes.
Recognition and killing of human and murine pancreatic beta cells by the NK receptor NKp46.
Proteolytic activation of the cytotoxic phenotype during human NK cell development.
NKp46 and NKG2D recognition of infected dendritic cells is necessary for NK cell activation in the human response to influenza infection.
Zhang H, Huang C, Chen X, Li L, Liu S, Li Y, Zhang Y, Zeng Y, Hu L
Genes & diseases 2020 Jun;7(2):283-289
Genes & diseases 2020 Jun;7(2):283-289
Induction of the Lytic Cycle Sensitizes Epstein-Barr Virus-Infected B Cells to NK Cell Killing That Is Counteracted by Virus-Mediated NK Cell Evasion Mechanisms in the Late Lytic Cycle.
Williams LR, Quinn LL, Rowe M, Zuo J
Journal of virology 2016 Jan 15;90(2):947-58
Journal of virology 2016 Jan 15;90(2):947-58
Plasmacytoid dendritic cells and C1q differentially regulate inflammatory gene induction by lupus immune complexes.
Santer DM, Wiedeman AE, Teal TH, Ghosh P, Elkon KB
Journal of immunology (Baltimore, Md. : 1950) 2012 Jan 15;188(2):902-15
Journal of immunology (Baltimore, Md. : 1950) 2012 Jan 15;188(2):902-15
Recognition and killing of human and murine pancreatic beta cells by the NK receptor NKp46.
Gur C, Enk J, Kassem SA, Suissa Y, Magenheim J, Stolovich-Rain M, Nir T, Achdout H, Glaser B, Shapiro J, Naparstek Y, Porgador A, Dor Y, Mandelboim O
Journal of immunology (Baltimore, Md. : 1950) 2011 Sep 15;187(6):3096-103
Journal of immunology (Baltimore, Md. : 1950) 2011 Sep 15;187(6):3096-103
Proteolytic activation of the cytotoxic phenotype during human NK cell development.
Meade JL, Wilson EB, Holmes TD, de Wynter EA, Brett P, Straszynski L, Ballard PA, Trapani JA, McDermott MF, Cook GP
Journal of immunology (Baltimore, Md. : 1950) 2009 Jul 15;183(2):803-13
Journal of immunology (Baltimore, Md. : 1950) 2009 Jul 15;183(2):803-13
NKp46 and NKG2D recognition of infected dendritic cells is necessary for NK cell activation in the human response to influenza infection.
Draghi M, Pashine A, Sanjanwala B, Gendzekhadze K, Cantoni C, Cosman D, Moretta A, Valiante NM, Parham P
Journal of immunology (Baltimore, Md. : 1950) 2007 Mar 1;178(5):2688-98
Journal of immunology (Baltimore, Md. : 1950) 2007 Mar 1;178(5):2688-98
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD56 (NCAM) PE (Product # 12-0567-42) and Mouse IgG1 K Isotype Control APC (Product # 17-4714-81) (left) or Anti-Human CD335 (NKp46) APC (right). Cells in the lymphocyte gate were used for analysis.