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- Antigen structure
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- Product number
- PA5-102860 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NKp46 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Antibody detects endogenous levels of total NCR1.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Tissue origin of cytotoxic natural killer cells dictates their differential roles in mouse digit tip regeneration and progenitor cell survival.
Dastagir N, Beal Z, Godwin J
Stem cell reports 2022 Mar 8;17(3):633-648
Stem cell reports 2022 Mar 8;17(3):633-648
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of NKp46 in 293 cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with NKp46 polyclonal antibody (Product # PA5-102860) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 P3-level digit tip amputations on C57Bl6/J mice show migration of natural killer (NK) cells to the wound site (A) Histological depiction of mouse P2 and P3 bones. The surgery site is indicated by a green dashed line. Tissue taken for FC and histology is indicated by a black dashed line. (B) Early time course of digit tip regeneration using trichrome staining. (C) Representative confocal microscopy with dual staining of PDGFRalpha + (MSCs) and NKP46 + (NK cell) invasion at each time point. (i) and (ii) show high magnification insets. Samples were counterstained with DAPI. White and blue dashed lines mark the nail and soft tissue and bone boundaries, respectively. n = 3; scale bars, 250 mum. (D) Quantification of NK cell recruitment to the wound via FC. NK cells (red gate) defined as CD3-negative and NK1.1-positive peak at 12 DPA and subsequently decrease by 15 DPA. n = 20 digits, five mice/condition. DPA, days post-amputation
