Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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Validation data
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- Product number
- PA5-27386 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Apolipoprotein D Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2. Predicted reactivity: Rabbit (81%), Bovine (83%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.18 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references LCAT, ApoD, and ApoA1 Expression and Review of Cholesterol Deposition in the Cornea.
Flores R, Jin X, Chang J, Zhang C, Cogan DG, Schaefer EJ, Kruth HS
Biomolecules 2019 Nov 26;9(12)
Biomolecules 2019 Nov 26;9(12)
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Apolipoprotein D Polyclonal Antibody detects Apolipoprotein D protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Apolipoprotein D protein stained by Apolipoprotein D Polyclonal Antibody (Product # PA5-27386) diluted at 1:500. Blue: Hoechst 33342 staining.