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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-27386 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Apolipoprotein D Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2.
- Concentration
- 0.68 mg/mL
Submitted references LCAT, ApoD, and ApoA1 Expression and Review of Cholesterol Deposition in the Cornea.
Flores R, Jin X, Chang J, Zhang C, Cogan DG, Schaefer EJ, Kruth HS
Biomolecules 2019 Nov 26;9(12)
Biomolecules 2019 Nov 26;9(12)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Apolipo D in Various whole cell extracts (30 µg). Samples were separated by 12% SDS-PAGE and the membrane was probed with Apolipoprotein D Polyclonal antibody (Product # PA5-27386) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Apolipoprotein D using 50 µg of A) mouse lung (B) mouse white adipose (C) mouse brown adipose and D) mouse stomach lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with an Apolipoprotein D polyclonal antibody (Product # PA5-27386) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Apolipoprotein D Polyclonal Antibody detects Apolipoprotein D protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Apolipoprotein D Polyclonal Antibody (Product # PA5-27386) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Apolipoprotein D Polyclonal Antibody detects Apolipoprotein D protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Apolipoprotein D protein stained by Apolipoprotein D Polyclonal Antibody (Product # PA5-27386) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded rat liver using Apolipoprotein D antibody (Product # PA5-27386) diluted 1:500. Apolipoprotein D protein is detected in the cytoplasm. Citrate buffer (pH 6.0 for 15 minutes) was used for antigen retrieval.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded mouse brown adipocyte using Apolipoprotein D antibody (Product # PA5-27386) diluted 1:500. Apolipoprotein D protein is detected in the cytoplasm. Citrate buffer (pH 6.0 for 15 minutes) was used for antigen retrieval.
