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LearnMA5-24246
antibody from Invitrogen Antibodies
Targeting: LAMA3
BM600-150kDa, epiligrin, kalinin-165kDa, LAMNA, nicein-150kDa
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- MA5-24246 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Laminin alpha-3 Monoclonal Antibody (546215)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs, no cross reactivity with recombinant human Laminin alpha 1, beta 1, gamma 2, or recombinant mouse Laminin alpha 4 is observed. Reconstitute at 0.5 mg/mL in sterile PBS.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 546215
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Laminin alpha-3 in human lung tissue. Samples were incubated in Laminin alpha-3 monoclonal antibody (Product # MA5-24246) using a dilution of 2 µg/mL followed by a HRP-conjugated Anti-Mouse IgG secondary antibody. A specific band was detected for Laminin α3/Laminin‚5 at approximately 190 kDa (as indicated). This experiment was conducted under non-reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of Laminin alpha-3 in immersion fixed NHEK human normal epidermal keratinocytes treated with 10 ng/mL Recombinant Human TGF-beta 1 for 24 hours. Samples were incubated in Laminin alpha-3 monoclonal antibody (Product # MA5-24246) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to the perinuclear space.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Laminin alpha-3 in immersion fixed paraffin-embedded sections of human stomach. Samples were incubated with Laminin alpha-3 monoclonal antibody (Product # MA5-24246) using a dilution of 5 µg/mL for 1 hour at room temperature followed by Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic . Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface of epithelial cells in gastric glands.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of Laminin alpha-3 in U2OS human osteosarcoma cell line. Samples were incubated in Laminin alpha-3 monoclonal antibody (Product # MA5-24246) or isotype control antibody followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody. To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer and permeabilized with Flow Cytometry Permeabilization/Wash Buffer.
