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Learn711306
antibody from Invitrogen Antibodies
Targeting: LAMA3
BM600-150kDa, epiligrin, kalinin-165kDa, LAMNA, nicein-150kDa
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
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- Product number
- 711306 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Laminin-332 Recombinant Polyclonal Antibody (5HCLC)
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 5HCLC
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis was performed on fixed and permeabilized A549 cells for detection of endogenous Laminin 332 using Anti-Laminin 322 Recombinant Rabbit Polyclonal Antibody (Product # 711306, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2,000). Panel a) shows representative cells that were stained for detection and localization of Laminin 332 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating localization of Laminin 332 in the extracellular matrix. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
