HPA023095

antibody from Atlas Antibodies

Targeting: HID1 C17orf28, DMC1, HID-1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA023095

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA023095, RRID:AB_1847766

Product name

Anti-HID1

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

RPSTSSASGQWSPTPEWVLSWKSKLPLQTIMRLLQ
VLVPQVEKICIDKGLTDESEILRFLQHGTLVGLLP
VPHPILIRKYQANSGTAM


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Western blot analysis in human cell lines MCF-7 and SK-MEL-30 using Anti-HID1 antibody. Corresponding HID1 RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.

Orthogonal method

Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.

Show more

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG spLane 4: Human plasma (IgG/HSA depleted)

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human esophagus shows strong cytoplasmic positivity in superficial squamous epithelial cells.

Sample type

HUMAN