21174-1-AP

antibody from Proteintech Group

Targeting: HID1 C17orf28, DMC1, HID-1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

21174-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#21174-1-AP, RRID:AB_10733124

Product name

C17orf28 antibody

Antibody type

Polyclonal

Description

KD/KO validated C17orf28 antibody (Cat. #21174-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Proteomic analysis of insulin secretory granules in INS-1 cells by protein correlation profiling.

Li M, Du W, Zhou M, Zheng L, Song E, Hou J
Biophysics reports 2018;4(6):329-338

---

HID-1 is required for homotypic fusion of immature secretory granules during maturation.

Du W, Zhou M, Zhao W, Cheng D, Wang L, Lu J, Song E, Feng W, Xue Y, Xu P, Xu T
eLife 2016 Oct 18;5

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

human brain tissue were subjected to SDS PAGE followed by western blot with 21174-1-AP(C17orf28 antibody) at dilution of 1:1000

Sample type

tissue

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using C17orf28 antibody 21174-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Sample type

cell line

Immunoprecipitation

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

IP Result of anti-C17orf28 (IP:21174-1-AP, 3ug; Detection:21174-1-AP 1:500) with mouse brain tissue lysate 10000ug.

Sample type

tissue