MA3-043

antibody from Invitrogen Antibodies

Targeting: CHRNA1 CHRNA

Western blot

Immunoprecipitation

Immunohistochemistry

Antibody data

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Validation data

Product number: MA3-043 - Provider product page
Provider: Invitrogen Antibodies
Product name: CHRNA1 Monoclonal Antibody (88B)
Antibody type: Monoclonal
Antigen: Purifed from natural sources
Description: MA3-043 detects nicotinic acetylcholine receptor (nAChR) gamma and delta subunits in torpedo and the delta subunit in mouse, human, rat, chicken and frog. This antibody does not detect the alpha 1 and beta 1 subunits. MA3-043 has been successfully used in Western blot, immunohistochemistry and immunoprecipitation procedures. By Western blot, this antibody detects a 60 kDa protein representing the gamma subunit and a 65 kDa protein representing the delta subunit from torpedo skeletal muscle homogenates. Under non-reducing conditions the delta subunit migrates mostly as a dimer of ~130 kDa. Immunohistochemical staining of nAChR in rat skeletal muscle with MA3-043 results in strong staining of the motor endplate. In Immunohistochemical staining, cryostat or permeabilization of the sections is recommended due to the reactivity of this antibody with the cytoplasmic side of the receptor. The MA3-043 antigen is purified Torpedo californica acetylcholine receptor.
Reactivity: Human, Mouse, Rat, Chicken/Avian
Host: Mouse
Isotype: IgG
Antibody clone number: 88B
Vial size: 100 µL
Concentration: Conc. Not Determined
Storage: -20° C, Avoid Freeze/Thaw Cycles

CHRNA1 protein structure - MA3-043 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Nicotinic Acetylcholine Receptor using Anti-Nicotinic Acetylcholine Receptor Monoclonal Antibody (88B) (Product # MA3-043) shows staining in Hela Cells. Nicotinic Acetylcholine Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Nicotinic Acetylcholine Receptor (Product # MA3-043) at a dilution of 1:100 over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503, Goat Anti-Mouse). Images were taken at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Nicotinic Acetylcholine Receptor using Anti-Nicotinic Acetylcholine Receptor Monoclonal Antibody (88B) (Product # MA3-043) shows staining in Neuro-2a Cells. Nicotinic Acetylcholine Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Nicotinic Acetylcholine Receptor (Product # MA3-043) at a dilution of 1:100 over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503, Goat Anti-Mouse). Images were taken at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Nicotinic Acetylcholine Receptor using Anti-Nicotinic Acetylcholine Receptor Monoclonal Antibody (88B) (Product # MA3-043) shows staining in U251 Cells. Nicotinic Acetylcholine Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Nicotinic Acetylcholine Receptor (Product # MA3-043) at a dilution of 1:20 over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503, Goat Anti-Mouse). Images were taken at 60X magnification.