Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AF136 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Angiopoietin-like Protein 3/ANGPTL3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse Angiopoietin-like Protein 3/ANGPTL3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) Angiopoietin-1, rhAngiopoietin-2, recombinant mouse Angiopoietin-3, rhAngiopoietin-4, and rhAngiopoietin-like factor/CDT6 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Furin is the primary in vivo convertase of angiopoietin-like 3 and endothelial lipase in hepatocytes.
Angiopoietin-like protein 3 regulates the motility and permeability of podocytes by altering nephrin expression in vitro.
Fetal liver hepatic progenitors are supportive stromal cells for hematopoietic stem cells.
Essalmani R, Susan-Resiga D, Chamberland A, Asselin MC, Canuel M, Constam D, Creemers JW, Day R, Gauthier D, Prat A, Seidah NG
The Journal of biological chemistry 2013 Sep 13;288(37):26410-8
The Journal of biological chemistry 2013 Sep 13;288(37):26410-8
Angiopoietin-like protein 3 regulates the motility and permeability of podocytes by altering nephrin expression in vitro.
Gao X, Xu H, Liu H, Rao J, Li Y, Zha X
Biochemical and biophysical research communications 2010 Aug 13;399(1):31-6
Biochemical and biophysical research communications 2010 Aug 13;399(1):31-6
Fetal liver hepatic progenitors are supportive stromal cells for hematopoietic stem cells.
Chou S, Lodish HF
Proceedings of the National Academy of Sciences of the United States of America 2010 Apr 27;107(17):7799-804
Proceedings of the National Academy of Sciences of the United States of America 2010 Apr 27;107(17):7799-804
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Mouse Angiopoietin-like Protein 3/ANGPTL3 by Western Blot. Western blot shows lysates of mouse liver tissue and Hepa 1-6 mouse hepatoma cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse Angiopoietin-like Protein 3/ANGPTL3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF136) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for Angiopoietin-like Protein 3/ANGPTL3 at approximately 35 kDa (major band) and 52 & 63 kDa (minor bands, as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Angiopoietin-like 3 in Mouse Liver. Angiopoietin-like 3 was detected in perfusion fixed frozen sections of mouse liver using Goat Anti-Mouse Angiopoietin-like 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF136) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of hepatocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.