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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
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- Product number
- PA5-28058 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ANGPTL3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HepG2 conditioned medium.
- Concentration
- 1.11 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ANGPTL3 using 30 µg of HepG2 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with an ANGPTL3 polyclonal antibody (Product # PA5-28058) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ANGPTL3 was performed by separating 10 µg of HepG2 conditioned medium by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a ANGPTL3 Polyclonal Antibody (Product # PA5-28058) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ANGPTL3 Polyclonal Antibody (Product # PA5-28058). HepG2 whole cell extract and conditioned medium (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with ANGPTL3 Polyclonal Antibody (Product # PA5-28058) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human hepatoma, using ANGPTL3 (Product # PA5-28058) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
