MA5-34646

antibody from Invitrogen Antibodies

Targeting: MAPRE3 EB3, RP3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-34646

Provider

Invitrogen Antibodies

Product name

MAPRE3 Recombinant Rabbit Monoclonal Antibody (JU38-30)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Positive Control: A431, PC-3M, SH-SY-5Y, rat cerebellum tissue, human lung cancer tissue, mouse brain tissue, mouse fallopian tubes tissue, K562.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

JU38-30

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles, store in dark

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of EB3 in Lane 1: Mouse brain tissue, Lane 2: K562. Samples were incubated with EB3 monoclonal antibody (Product # MA5-34646), at a dilution of 1:500.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of EB3 in SH-SY-5Y cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with EB3 monoclonal antibody (Product # MA5-34646), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of EB3 in PC-3M cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with EB3 monoclonal antibody (Product # MA5-34646), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of EB3 in A431 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with EB3 monoclonal antibody (Product # MA5-34646), followed by DAPI (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of EB3 in paraffin-embedded mouse fallopian tubes tissue. Samples were incubated with EB3 monoclonal antibody (Product # MA5-34646), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of EB3 in paraffin-embedded rat cerebellum tissue. Samples were incubated with EB3 monoclonal antibody (Product # MA5-34646), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of EB3 in paraffin-embedded mouse fallopian tubes tissue. Samples were incubated with EB3 monoclonal antibody (Product # MA5-34646), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of EB3 in paraffin-embedded mouse brain tissue. Samples were incubated with EB3 monoclonal antibody (Product # MA5-34646), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of EB3 in paraffin-embedded human lung cancer tissue. Samples were incubated with EB3 monoclonal antibody (Product # MA5-34646), and followed by hematoxylin.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of EB3 in SH-SY-5Y cells (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Samples were incubated with EB3 monoclonal antibody (Product # MA5-34646) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.