AF3166

antibody from Novus Biologicals

Targeting: FABP7 B-FABP, BLBP

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF3166

Provider

Novus Biologicals

Product name

Goat Polyclonal FABP7/B-FABP Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human FABP7/B-FABP in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) FABP1, rhFABP2, rhFABP3, rhFABP4, rhFABP5, rhFABP6, rhFABP8, rhFABP9 and recombinant mouse FABP9 is observed.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Meis1 Coordinates Cerebellar Granule Cell Development by Regulating Pax6 Transcription, BMP Signaling and Atoh1 Degradation.

Owa T, Taya S, Miyashita S, Yamashita M, Adachi T, Yamada K, Yokoyama M, Aida S, Nishioka T, Inoue YU, Goitsuka R, Nakamura T, Inoue T, Kaibuchi K, Hoshino M
The Journal of neuroscience : the official journal of the Society for Neuroscience 2018 Jan 31;38(5):1277-1294

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Functional analysis of fatty acid binding protein 7 and its effect on fatty acid of renal cell carcinoma cell lines.

Takaoka N, Takayama T, Ozono S
BMC cancer 2017 Mar 14;17(1):192

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Expression of fatty acid-binding proteins in adult hippocampal neurogenic niche of postischemic monkeys.

Boneva NB, Kaplamadzhiev DB, Sahara S, Kikuchi H, Pyko IV, Kikuchi M, Tonchev AB, Yamashima T
Hippocampus 2011 Feb;21(2):162-71

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human FABP7/B-FABP by Simple Western^TM. Simple Western lane view shows lysates of human cerebellum tissue and human hippocampus tissue, loaded at 0.2 mg/mL. A specific band was detected for FABP7/B-FABP at approximately 18 kDa (as indicated) using 50 µg/mL of Goat Anti-Human FABP7/B-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3166) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

FABP7 in Human Melanoma. FABP7 was detected in immersion fixed paraffin-embedded sections of human melanoma using 5 µg/mL Goat Anti-Human FABP7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3166) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.