PA5-117944

antibody from Invitrogen Antibodies

Targeting: FABP7 B-FABP, BLBP

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-117944

Provider

Invitrogen Antibodies

Product name

FABP7 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

FABP7 Immunoprecipitation using: Lane A: 0.5 mg U251MG Whole Cell Lysate 4 µL with FABP7 Polyclonal Antibody (Product # PA5-117944) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: FABP7 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) /HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 15 kDa. Observed band size: 15 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of FABP7 in Lane A: 0.5 mg U251MG Whole Cell Lysate. Samples were treated with 60 μg of immunomagnetic Protein A/G beads, incubated with polyclonal antibody (Product # PA5-117944) with a dilution of 1:100 , followed by Goat Anti-Rabbit IgG (H+L)/HRP using a dilution of 1:10,000. Assay was performed under reducing conditions. Predicted band size: 15 kDa , Observed band size : 15 kDa .

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) of FABP7 in human malignant melanoma. Samples were incubated with polyclonal antibody (Product # PA5-117944) with a dilution of 1:5,000 .

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) of FABP7 in human brain. Samples were incubated with polyclonal antibody (Product # PA5-117944) with a dilution of 1:5,000 .

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

FABP7 Immunoprecipitation using: Lane A: 0.5 mg U251MG Whole Cell Lysate 4 µL with FABP7 Polyclonal Antibody (Product # PA5-117944) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: FABP7 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) /HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 15 kDa. Observed band size: 15 kDa.