Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [8]
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Validation data
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- Product number
- PA5-52661 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LHPP Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: SGVLYDSGAG GGTAIAGSVE AVARLKRSRL KVRFCTNESQ KSRAELVGQL QRLGFDISEQ EVTAPAPAAC QILKERGLRP YLLIHDGVRS EFDQIDTSNP NCVVIADAGE Highest antigen sequence identity to the following orthologs: Mouse - 85%, Rat - 90%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of LHPP in human cell lines A-431 and Caco-2 using a LHPP Polyclonal Antibody (Product # PA5-52661). Corresponding LHPP RNA-seq data are presented for the same cell lines. Loading control: Anti-PPIB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of LHPP in human cell line U-2 OS shows positivity in nucleus & cytoplasm. Samples were probed using a LHPP Polyclonal Antibody (Product # PA5-52661).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemical staining of LHPP in human skeletal muscle using LHPP Polyclonal Antibody (Product # PA5-52661) shows no positivity in myocytes as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of LHPP in human testis using LHPP Polyclonal Antibody (Product # PA5-52661).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of LHPP in human cerebral cortex using a LHPP Polyclonal Antibody (Product # PA5-52661) shows strong nuclear and cytoplasmic positivity in glial cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of LHPP in human colon, kidney, liver and testis using LHPP Polyclonal Antibody (Product # PA5-52661) (A) shows similar protein distribution across tissues to an independent LHPP Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of LHPP in human liver using LHPP Polyclonal Antibody (Product # PA5-52661) shows moderate cytoplasmic positivity in hepatocytes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of LHPP in human colon using LHPP Polyclonal Antibody (Product # PA5-52661) shows positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of LHPP in human kidney using LHPP Polyclonal Antibody (Product # PA5-52661) shows moderate cytoplasmic positivity in cells in tubules.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of LHPP in human cerebral cortex using LHPP Polyclonal Antibody (Product # PA5-52661) shows moderate cytoplasmic positivity.