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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- RQ8608 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- LHPP Antibody / Phospholysine phosphohistidine inorganic pyrophosphate phosphatase
- Antibody type
- Polyclonal
- Description
- This highly specific LHPP antibody is suitable for use in Western blot/Immunofluorescence/Flow cytometry/ELISA applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the LHPP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human A549, 2) human HeLa, 3) human A431, 4) human HepG2, 5) human HaCaT, 6) human MCF7, 7) human HEL, 8) rat RH35 and 9) mouse HEPA1-6 cell lysate with LHPP antibody. Predicted molecular weight ~29 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human U-2 OS cells with LHPP antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of fixed and permeabilized human JK cells with LHPP antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= LHPP antibody.
