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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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- Product number
- PA5-49333 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Adenylate Kinase 4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of AK4 in lysates from A431 (35 µg), Hela (35 µg), HepG2 (35 µg), MCF-7 (35 µg), Raji cell line (35 µg) and mouse kidney tissue lysate (35 µg) (from left to right). Samples were probed with an AK4 Antibody (Center) (Product # PA5-49333) diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorecent analysis of AK4 in HepG2 cells. Cells were stained with AK4 polyclonal Antibody (Center) (Product # PA5-49333) at a 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclei (blue). Cytoplasmic actin was counterstained with Alexa Fluor 555 Phalloidin (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of AK4 in paraffin-embedded Human kidney tissue. Samples were probed with a AK4 Antibody at a 1:25 dilution (Product # PA5-49333) followed by peroxidase conjugation to the goat anti-rabbit secondary antibody IgG at 1:400 dilution and DAB staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of AK4 in paraffin-embedded Human heart section. Samples were probed with an AK4 Antibody (Center) (Product # PA5-49333) at a 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of AK4 in paraffin-embedded Mouse kidney section. Samples were incubated using AK4 Antibody (Center) (Product # PA5-49333) at a 1:25 dilution, followed by a peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
