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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Flow cytometry [2]
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Validation data
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- Product number
- TA502910 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA502910, RRID:AB_11128602
- Product name
- AK4 mouse monoclonal antibody, clone OTI3E1 (formerly 3E1)
- Antibody type
- Monoclonal
- Description
- AK4 mouse monoclonal antibody, clone OTI3E1 (formerly 3E1)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- AK4
- Isotype
- IgG
- Antibody clone number
- OTI3E1
- Vial size
- 100 µl
- Concentration
- 0.68 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AK4 (RC220572, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AK4.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-AK4 monoclonal antibody.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC220572 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-AK4 antibody(TA502910), and then analyzed by flow cytometry.
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Hela cells, using anti-AK4 antibody(TA502910),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
- Validation comment
- FC
