Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-36544 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL-11 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Purity is >95% by SDS-PAGE.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references A myeloid-stromal niche and gp130 rescue in NOD2-driven Crohn's disease.
Transgenic interleukin 11 expression causes cross-tissue fibro-inflammation and an inflammatory bowel phenotype in mice.
Nayar S, Morrison JK, Giri M, Gettler K, Chuang LS, Walker LA, Ko HM, Kenigsberg E, Kugathasan S, Merad M, Chu J, Cho JH
Nature 2021 May;593(7858):275-281
Nature 2021 May;593(7858):275-281
Transgenic interleukin 11 expression causes cross-tissue fibro-inflammation and an inflammatory bowel phenotype in mice.
Lim WW, Ng B, Widjaja A, Xie C, Su L, Ko N, Lim SY, Kwek XY, Lim S, Cook SA, Schafer S
PloS one 2020;15(1):e0227505
PloS one 2020;15(1):e0227505
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of IL-11 in paraffin-embedded human breast carcinoma tissue. Samples were incubated with IL-11 polyclonal antibody (Product # PA5-36544) at a dilution of 1:100.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig 2 Il11 expression results in fibro-inflammatory disease of the colon. (a) Representative images of the Il11 SMC mice before (d0) and up to 7 days (d7) treatment with either corn oil vehicle (veh) or tamoxifen (tam). Presence of rectal prolapse are indicated with white arrows. Images represent the same animal across time points not taken to the same scale. (b) Excised gastrointestinal tract of representative Il11 SMC mice at day 14 post-treatment with veh or tam. Scale bar represents 5 cm. (c) Fecal calprotectin in representative Il11 SMC mice treated with veh or tam assessed by ELISA ( n = 6-8 per group). (d) Representative cross-section and longitudinal section of the colon stained with Masson's trichrome (left) and at 200X magnification (right). Scale bar of cross and longitudinal sections represents 500 mum and at 200X magnification represents 200 mum. (e) Colon fibrosis determined as a percentage of collagen positive area (blue) from histological images taken at 200X magnification ( n = 6 per group). (f) Tunica muscularis (smooth muscle) thickness of the colon ( n = 6 per group). (g) Total collagen content assessed by hydroxyproline assay and expressed as fold change (FC) of veh-treated Il11 SMC mice ( n = 10-11 per group). (h) Representative images of the colonic smooth muscle and crypts taken at 400X magnification for Masson's trichrome and immunohistochemistry staining for IL11, cluster of differentiation 45 (CD45), lysosome-associated membrane protein 2 (LAMP2), a