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- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- ABIN359726 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Chloride Intracellular Channel 4 (CLIC4) antibody
- Antibody type
- Polyclonal
- Antigen
- This antibody is generated from rabbits immunized with recombinant human CLIC4 protein.
- Description
- Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.4 mL
- Concentration
- 0.25 mg/mL
- Storage
- Store the antibody undiluted at 2-8°C for one month or (in aliquots) at-20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Reciprocal modifications of CLIC4 in tumor epithelium and stroma mark malignant progression of multiple human cancers.
Functional reconstitution of mammalian 'chloride intracellular channels' CLIC1, CLIC4 and CLIC5 reveals differential regulation by cytoskeletal actin.
Localization of calcium in the smooth endoplasmic reticulum of rat isolated fat cells.
Suh KS, Crutchley JM, Koochek A, Ryscavage A, Bhat K, Tanaka T, Oshima A, Fitzgerald P, Yuspa SH
Clinical cancer research : an official journal of the American Association for Cancer Research 2007 Jan 1;13(1):121-31
Clinical cancer research : an official journal of the American Association for Cancer Research 2007 Jan 1;13(1):121-31
Functional reconstitution of mammalian 'chloride intracellular channels' CLIC1, CLIC4 and CLIC5 reveals differential regulation by cytoskeletal actin.
Singh H, Cousin MA, Ashley RH
The FEBS journal 2007 Dec;274(24):6306-16
The FEBS journal 2007 Dec;274(24):6306-16
Localization of calcium in the smooth endoplasmic reticulum of rat isolated fat cells.
Hales CN, Luzio JP, Chandler JA, Herman L
Journal of cell science 1974 Jun;15(1):1-15
Journal of cell science 1974 Jun;15(1):1-15
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of CLIC4 (arrow) using rabbit polyclonal CLIC4 Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CLIC4 gene (Lane 2)
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with CLIC4 antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
