Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [8]
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Validation data
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- Product number
- NBP1-85574 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-85574, RRID:AB_11018841
- Product name
- Rabbit Polyclonal CLIC4 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human CLIC4 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Histamine H3 receptors aggravate cerebral ischaemic injury by histamine-independent mechanisms.
Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells.
Impact of genomic stability on protein expression in endometrioid endometrial cancer.
Yan H, Zhang X, Hu W, Ma J, Hou W, Zhang X, Wang X, Gao J, Shen Y, Lv J, Ohtsu H, Han F, Wang G, Chen Z
Nature communications 2014 Feb 25;5:3334
Nature communications 2014 Feb 25;5:3334
Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells.
Stadler C, Rexhepaj E, Singan VR, Murphy RF, Pepperkok R, Uhlén M, Simpson JC, Lundberg E
Nature methods 2013 Apr;10(4):315-23
Nature methods 2013 Apr;10(4):315-23
Impact of genomic stability on protein expression in endometrioid endometrial cancer.
Lomnytska MI, Becker S, Gemoll T, Lundgren C, Habermann J, Olsson A, Bodin I, Engström U, Hellman U, Hellman K, Hellström AC, Andersson S, Mints M, Auer G
British journal of cancer 2012 Mar 27;106(7):1297-305
British journal of cancer 2012 Mar 27;106(7):1297-305
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CLIC4 Antibody [NBP1-85574] - Analysis in human cell lines SK-MEL-30 and MCF-7 using anti-CLIC4 antibody. Corresponding CLIC4 RNA-seq data are presented for the same cell lines. Loading control: anti-PFN1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CLIC4 Antibody [NBP1-85574] - Staining of human kidney shows high expression.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CLIC4 Antibody [NBP1-85574] - Staining of human skeletal muscle shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CLIC4 Antibody [NBP1-85574] - Staining in human kidney and skeletal muscle tissues using anti-CLIC4 antibody. Corresponding CLIC4 RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CLIC4 Antibody [NBP1-85574] - Staining of human colon using Anti-CLIC4 antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CLIC4 Antibody [NBP1-85574] - Human endometrium using Anti-CLIC4 antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CLIC4 Antibody [NBP1-85574] - Staining of human colon, endometrium, kidney and skeletal muscle using Anti-CLIC4 antibody NBP1-85574 (A) shows similar protein distribution across tissues to independent antibody NBP2-49321 (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CLIC4 Antibody [NBP1-85574] - Staining of human skeletal muscle using Anti-CLIC4 antibody NBP1-85574.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CLIC4 Antibody [NBP1-85574] - Staining of human kidney using Anti-CLIC4 antibody NBP1-85574.