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LearnPA5-28146
antibody from Invitrogen Antibodies
Targeting: CLIC4
CLIC4L, DKFZP566G223, H1, huH1, P64H1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
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- Product number
- PA5-28146 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CLIC4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa, Raji. Predicted reactivity: Mouse (98%), Rat (96%), Zebrafish (85%), Xenopus laevis (87%), Rhesus Monkey (99%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.83 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CLIC4 using 30 µg of Raji lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a CLIC4 polyclonal antibody (Product # PA5-28146) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CLIC4 was performed by separating 30 µg of various whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a CLIC4 Polyclonal Antibody (Product # PA5-28146) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CLIC4 was performed by separating 30 µg of various whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a CLIC4 Polyclonal Antibody (Product # PA5-28146) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of CLIC4 in paraformaldehyde-fixed HeLa cells using a CLIC4 polyclonal antibody (Product # PA5-28146) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2500.
