PA5-109604

antibody from Invitrogen Antibodies

Targeting: CTSE

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-109604

Provider

Invitrogen Antibodies

Product name

Cathepsin E Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: GQSFSIQYGT GSLSGIIGAD QVSVEGLTVV GQQFGESVTE PGQTFVDAEF DGILGLGYPS LAVGGVTPVF DNMMAQNLVD LPMFSVYMSS NPEGGAGSEL IFGGYDHSHF SGS

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.66 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Cathepsin E in extracts of various cell lines. Samples were incubated with Cathepsin E Polyclonal antibody (Product # PA5-109604) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of Cathepsin E in MCF-7. Samples were incubated in polyclonal Cathepsin E antibody (Product # PA5-109604) using a dilution of 1:100.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Cathepsin E in paraffin-embedded human gastric cancer. Samples were incubated with Cathepsin E Polyclonal antibody (Product # PA5-109604) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Cathepsin E in paraffin-embedded human tonsil. Samples were incubated with Cathepsin E Polyclonal antibody (Product # PA5-109604) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Cathepsin E in paraffin-embedded human lung. Samples were incubated with Cathepsin E Polyclonal antibody (Product # PA5-109604) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Cathepsin E in paraffin-embedded human tonsil. Samples were incubated with Cathepsin E Polyclonal antibody (Product # PA5-109604) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Cathepsin E in paraffin-embedded human lung. Samples were incubated with Cathepsin E Polyclonal antibody (Product # PA5-109604) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Cathepsin E in paraffin-embedded human gastric cancer. Samples were incubated with Cathepsin E Polyclonal antibody (Product # PA5-109604) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.