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LearnPA5-21514
antibody from Invitrogen Antibodies
Targeting: LAMB3
BM600-125kDa, kalinin-140kDa, LAMNB1, nicein-125kDa
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-21514 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Laminin beta-3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A431, NIH-3T3, mouse bone marrow. Predicted reactivity: Mouse (81%), Rat (80%), Bovine (80%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.46 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Mutations in collagen, type XVII, alpha 1 (COL17A1) cause epithelial recurrent erosion dystrophy (ERED).
Jonsson F, Byström B, Davidson AE, Backman LJ, Kellgren TG, Tuft SJ, Koskela T, Rydén P, Sandgren O, Danielson P, Hardcastle AJ, Golovleva I
Human mutation 2015 Apr;36(4):463-73
Human mutation 2015 Apr;36(4):463-73
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Laminin beta-3 using 30 µg of A431 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a Laminin beta-3 polyclonal antibody (Product # PA5-21514) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Laminin beta-3 was performed by separating 30 µg of whole cell extract by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a Laminin beta-3 Polyclonal Antibody (Product # PA5-21514) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Laminin beta-3 Polyclonal Antibody (Product # PA5-21514). Sample (30 µg of whole cell lysate). Lane A: NIH-3T3. 7.5% SDS PAGE. Laminin beta-3 Polyclonal Antibody (Product # PA5-21514) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of Laminin-beta-3 was performed in A431 cells fixed in 4% paraformaldehyde at RT for 15 min. Green: Laminin beta-3 Polyclonal Antibody (Product # PA5-21514) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human lung cancer, using Laminin beta 3 (Product # PA5-21514) antibody at 1:250 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
