Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-62904 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ZNF580 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Immunogen sequence: PRSSSPEAMD PPPPKAPPFP KAEGPSSTPS SAAGPRPPRL GRHLLIDANG Highest antigen sequence identity to the following orthologs: Mouse - 92%, Rat - 92%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.4 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Role and mechanism of the zinc finger protein ZNF580 in foam-cell formation.
Zhang Z, Qin X, Liu J, Li Y, Chen H, Xie H, Chen J, Li C, Tong Y, Yang M, Zhang M
Experimental and therapeutic medicine 2022 Sep;24(3):579
Experimental and therapeutic medicine 2022 Sep;24(3):579
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorecent analysis of ZNF580 in human cell line U-2 OS using ZNF580 Polyclonal Antibody (Product # PA5-62904). Staining shows localization to nucleus.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of ZNF580 in human Nasopharynx using ZNF580 Polyclonal Antibody (Product # PA5-62904) shows moderate nuclear positivity in respiratory epithelial cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 LV-ZNF580 and Si-ZNF580 lentivirus transfection. (A) Fluorescence microscopy was used to examine the transfection efficiency of LV-ZNF580 and Si-ZNF580 lentivirus (magnification, x200). Green fluorescence was visible in the cytoplasm and around the nucleus of transfected THP-1 cells. (B) The level of ZNF580 mRNA expression was measured by reverse transcription-quantitative PCR. (C) The levels of ZNF580 protein in THP-1 cells transfected with lentivirus were measured by western blot analysis. * P