ACR-060-25UL

antibody from Invitrogen Antibodies

Targeting: CALCRL CGRPR, CRLR

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

ACR-060-25UL

Provider

Invitrogen Antibodies

Product name

CRLR/CALCRL (extracellular) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

25 µL

Concentration

0.8 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of human THP-1 monocytic leukemia cell line lysate: - 1. Anti-CRLR/CALCRL (extracellular) Antibody (#ACR-060), (1:200). 2. Anti-CRLR/CALCRL (extracellular) Antibody , preincubated with CRLR/CALCRL (extracellular) Blocking Peptide (#BLP-CR060).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of rat dorsal root ganglion lysates (lanes 1 and 4), rat lung membranes (lanes 2 and 5) and rat heart membranes (lanes 3 and 6): - 1-3. Anti-CRLR/CALCRL (extracellular) Antibody (#ACR-060), (1:200). 4-6. Anti-CRLR/CALCRL (extracellular) Antibody , preincubated with CRLR/CALCRL (extracellular) Blocking Peptide (#BLP-CR060).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of mouse heart lysates (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4): - 1, 2. Anti-CRLR/CALCRL (extracellular) Antibody (#ACR-060), (1:200). 3, 4. Anti-CRLR/CALCRL (extracellular) Antibody , preincubated with CRLR/CALCRL (extracellular) Blocking Peptide (#BLP-CR060).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of human THP-1 monocytic leukemia cell line lysate: - 1. Anti-CRLR/CALCRL (extracellular) Antibody (#ACR-060), (1:200). 2. Anti-CRLR/CALCRL (extracellular) Antibody , preincubated with CRLR/CALCRL (extracellular) Blocking Peptide (#BLP-CR060).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expression of Calcitonin Receptor-Like Receptor in rat brain stem - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-CRLR/CALCRL (extracellular) Antibody (#ACR-060), (1:200), followed by goat Anti-rabbit-AlexaFluor-488. A. CRLR staining (green) in the Raphe Magnus region is detected in neurons (arrows). B. Pre-incubation of the Antibody with CRLR/CALCRL (extracellular)Blocking Peptide (#BLP-CR060), suppresses staining. Cell nuclei are stained with DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expression of Calcitonin Receptor-Like Receptor in mouse brain stem - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-CRLR/CALCRL (extracellular) (extracellular) Antibody (#ACR-060), (1:200), followed by goat Anti-rabbit-AlexaFluor-488. A. CRLR staining (green) in the Raphe Magnus region is detected in (green) in neurons (arrows). B. Pre-incubation of the Antibody with CRLR/CALCRL (extracellular)Blocking Peptide (#BLP-CR060), suppresses staining. Cell nuclei are stained with DAPI (blue).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Cell surface detection of CRLR in live intact human THP-1 monocytic leukemia cells: - (black line) cells. (red) Cells + goat- Anti-rabbit-FITC. (green) Cells + Anti-CRLR/CALCRL (extracellular) Antibody (#ACR-060), (2.5 µg) + goat- Anti-rabbit-FITC.