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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
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- Product number
- PA5-56315 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RECQL5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: ELEHETFRNA KVANLYKASV LKKVADIHRA SKDGQPYDMG GSAKSCSAQA EPPEPNEYDI PPASHVYSLK PKRVGAGFPK GSCPFQTATE LMETTRIREQ Highest antigen sequence identity to the following orthologs: Mouse - 73%, Rat - 72%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.7 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Deorphanizing Caspase-3 and Caspase-9 Substrates In and Out of Apoptosis with Deep Substrate Profiling.
Araya LE, Soni IV, Hardy JA, Julien O
ACS chemical biology 2021 Nov 19;16(11):2280-2296
ACS chemical biology 2021 Nov 19;16(11):2280-2296
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RECQL5 in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using a RECQL5 Polyclonal Antibody (Product # PA5-56315). Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of RECQL5 in human cell line A-431 shows positivity in nucleus & cytoplasm. Samples were probed using a RECQL5 Polyclonal Antibody (Product # PA5-56315).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RECQL5 in human placenta using RECQL5 Polyclonal Antibody (Product # PA5-56315) shows no positivity in stromal cells as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RECQL5 in human testis using RECQL5 Polyclonal Antibody (Product # PA5-56315) shows moderate to strong nuclear positivity in squamous epithelial cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RECQL5 in human cerebral cortex using RECQL5 Polyclonal Antibody (Product # PA5-56315) shows moderate to strong nuclear positivity in neurons.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RECQL5 in human cervix, uterine using RECQL5 Polyclonal Antibody (Product # PA5-56315) shows moderate to strong nuclear positivity in squamous epithelial cells.
