NBP1-40340

antibody from Novus Biologicals

Targeting: RECQL5 FLJ90603, RecQ5

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

NBP1-40340

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-40340, RRID:AB_2178436

Product name

Rabbit Polyclonal RECQL5 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 ul

Concentration

1.0 mg/ml

Storage

Store at 4C. Do not freeze.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: RECQL5 Antibody [NBP1-40340] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RECQ5 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 10 mcg/mg lysate. RECQ5 was also immunoprecipitated by rabbit anti-RECQ5 antibodies NBP1-40339 and NBP1-40341 which recognize other epitopes.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: RECQL5 Antibody [NBP1-40340] - Detection of Human RECQ5 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RECQ5 antibody NBP1-40340 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.